A model for individual and collective cell movement in Dictyostelium discoideum
A model for individual and collective cell movement in Dictyostelium discoideum
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DOI：
10.1073/pnas.97.19.10448
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发表时间：
2000-09-12
影响因子：
11.1
通讯作者：
Othmer, HG
中科院分区：
文献类型：
--
作者：
Palsson, E;Othmer, HG
盘基网柄黏菌（Dictyosteelium discoideum）是一种广泛应用的模式系统，用于研究发育过程中的各种基本过程，包括细胞-细胞信号传导、信号转导、模式形成、细胞运动等。以及组织样细胞聚集体的运动。细胞运动的许多方面都知之甚少，包括单个细胞的行为如何产生在丘和蛞蝓中观察到的细胞集体运动。在这里，我们描述了一个生物学上现实的模型，能动的D，discoideum细胞，可以产生积极的力量，通过表面分子相互作用，可以检测和响应趋化信号。我们将细胞建模为可变形的粘弹性椭圆体，并通过使用先前开发的模型将信号转导和细胞间信号传导结合起来。形状约束限制了容许的变形，但使得大量相互作用的细胞的模拟是可行的，因为该模型是基于已知的过程，参数可以估计或测量实验。我们表明，该模型可以重现观察到的单细胞的趋化行为，聚集过程中的流动，和一小群起搏器驱动的细胞聚集体的集体运动。该模型预测，运动的二维段塞[邦纳。J，T，（1998）Proc.Natl. Acad. Sci. USA 95，9355-9359]由单个细胞表现出的相同行为产生;没有必要调用不同的机制或行为。我们的计算实验还表明，以前未表征的现象，可能是实验观察。
The cellular slime mold Dictyostelium discoideum is a widely used model system for studying a variety of basic processes in development, including cell-cell signaling, signal transduction, pattern formation, cell motility. and the movement of tissue-like aggregates of cells. Many aspects of cell motion are poorly understood, including how individual cell behavior produces the collective motion of cells observed within the mound and slug. Herein, we describe a biologically realistic model for motile D, discoideum cells that can generate active forces, that interact via surface molecules, and that can detect and respond to chemotactic signals. We model the cells as deformable viscoelastic ellipsoids and incorporate signal transduction and cell-cell signaling by using a previously developed model. The shape constraint restricts the admissible deformations but makes the simulation of a large number of interacting cells feasible, Because the model is based on known processes, the parameters can be estimated or measured experimentally. We show that this model can reproduce the observations on the chemotactic behavior of single cells, streaming during aggregation, and the collective motion of an aggregate of cells driven by a small group of pacemakers. The model predicts that the motion of two-dimensional slugs [Bonner. J, T, (1998) Proc. Natl. Acad. Sci. USA 95, 9355-9359] results from the same behaviors that are exhibited by individual cells; it is not necessary to invoke different mechanisms or behaviors. Our computational experiments also suggest previously uncharacterized phenomena that may be experimentally observable.