Alkaline secretion by amphibian duodenum. III. Effect of DBcAMP, theophylline, and prostaglandins.
Alkaline secretion by amphibian duodenum. III. Effect of DBcAMP, theophylline, and prostaglandins.
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两栖动物十二指肠的碱性分泌。
DOI：
10.1152/ajpgi.1981.241.6.g528
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Silen,W
中科院分区：
文献类型：
--
作者：
Simson,JN;Merhav,A;Silen,W
材料与方法美国牛蛙（Rana catesbeiana）在4 ℃盐水中保存。脊髓切片后，切除十二指肠近端2 cm段，并去除浆肌层。将粘膜固定在l-cm 2暴露面积的Lucite室中，并在室温下用通过气升系统循环的溶液浸泡。营养物（丝氨酸）溶液含有（mM）：Na+，102.4; Cl-，91.4; K ′，4.0; Mg 2+，0.8; Ca 2 ′，1.8; H2 PO 4，0.8; SO 2-，0.8; HCO 2，17.8;和葡萄糖，2.0，并且用95%~ 25%CO2充气。等渗的、未缓冲的分泌（粘膜）溶液含有（mM）：Na+，102.4; Cl-，91.4; K+，4.0; Mg 2+，0.8; Ca 2+，1.8; S+，10.1;和甘露醇，11.3，并且用100%O2充气。通过在分泌液中取代S@-和在营养液中取代羟乙基磺酸盐来制备无Cl溶液。通过用胆碱代替Na+来制备无Na+溶液。用5 mM N-2-羟乙基哌嗪-N '-乙磺酸（HEPES）缓冲不含HCOF的溶液并用100%O2充气。通过用95%N2 - 5%CO2的营养液和用100%N2的分泌液充气来实现缺氧。营养盐pH值为7.23。在分泌液中测量碱性分泌
MATERIALS AND METHODSAmerican bullfrogs (Rana catesbeiana) were stored at 4OC in saline. After section of the spinal cord, the proximal 2-cm segment of duodenum was excised, and the seromuscular layers were removed. The mucosa was mounted in a Lucite chamber of l-cm2 exposed area and bathed by solutions at room temperature circulated by gas-lift systems. The nutrient (serosal) solution contained (mM): Na+, 102.4; Cl-, 91.4; K’, 4.0; Mg2+, 0.8; Ca2’, 1.8; H2P04, 0.8; SO:-, 0.8; HCO;, 17.8; and glucose, 2.0 and was gassed with 95% 025% COZ. The isosmolar, unbuffered secretory (mucosal) solution contained (mM): Na+, 102.4; Cl-, 91.4; K+, 4.0; Mg2+, 0.8; Ca2’, 1.8; S@, 10.1; and mannitol, 11.3 and was gassed with 100% 02. Cl-free solutions were made by substituting S@-in the secretory solution and isethionate in the nutrient solution. Na+-free solutions were made by substituting choline for Na+. HCOF-free solutions were buffered with 5 mM N-2-hydroxyethylpiperazine-N’-ethanesulfonic acid (HEPES) and gassed with 100% 02. Anoxia was achieved by gassing the nutrient solution with 95% N2-5% Co2 and the secretory solution with 100% N2. The nutrient pH was 7.23. Alkaline secretion was measured in the secretory solution