Clinical and histological healing of surgical wounds treated with mitomycin C
Clinical and histological healing of surgical wounds treated with mitomycin C
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DOI：
10.1097/00005537-200401000-00027
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发表时间：
2004-01-01
期刊：
影响因子：
2.6
通讯作者：
Eckley, CA
中科院分区：
文献类型：
--
作者：
Ribeiro, FDQ;Guaraldo, L;Eckley, CA
目标/假设。丝裂霉素C在体外抑制成纤维细胞的能力已被证明，这刺激了它在研究动物和人类中控制愈合的使用。该研究的目的是跟踪局部应用丝裂霉素C的大鼠背部手术伤口的愈合过程。研究设计：前瞻性实验研究。方法：研究方法。对10只成年大鼠背部造成两个不同的创面。其中一个创面在5分钟内外用0.5 mg/mL的丝裂霉素C，另一个创面作为对照。分别于治疗后不同时间处死大鼠，进行临床和组织学观察。纤维化程度由两位不同的病理专家进行组织学评估。结果：与未处理创面相比，应用丝裂霉素C的手术创面愈合延迟，对照创面7d结痂消失。治疗后1个月的组织学分析显示，与未治疗的伤口相比，丝裂霉素C治疗的伤口的纤维化程度显著减轻。第三个月后，两种创面的纤维化程度相似。结论：局部应用丝裂霉素C可将大鼠手术创面延迟至治疗后第4周愈合，但12周后治疗创面和未治疗创面的纤维化程度相当。在耳鼻咽喉科，该药物的这一特性可用于治疗外耳道狭窄、后鼻孔闭锁、鼻瘢痕狭窄、喉狭窄和瘢痕疙瘩。
Objectives/Hypothesis. The proven ability of mitomycin C to inhibit fibroblasts in vitro has stimulated its use in research animals and in humans to control healing. The objective of the study was to follow the healing process of surgical wounds in the dorsum of rats treated topically with mitomycin C. Study Design: Prospective experimental study. Methods. Two distinct surgical wounds were made to the dorsum of 10 adult rats. One of the wounds received topical mitomycin C diluted at 0.5 mg/mL during a 5-minute period, and the other wound was used as a control. The healing process was followed clinically and histologically after the rats were killed at different post-treatment periods. The degree of fibrosis was evaluated histologically by two different pathologists. Results: Surgical wounds treated with mitomycin C presented delayed healing when compared with the untreated wounds, with remission of scabs 7 days after the control wounds. Histological analysis at 1 month after treatment revealed a significant reduction in fibrosis of the wounds treated with mitomycin C when compared with the untreated wounds. After the third month the degree of fibrosis was comparable in both wounds. Conclusion: Topical mitomycin C delays the healing of surgical wounds in rats up to the fourth week following treatment, but the degree of fibrosis is comparable in both treated and untreated wounds after 12 weeks. In otolaryngology this characteristic of the drug may be useful in the treatment of external ear canal stenosis, choanal atresias, nasal cicatricial stenosis, laryngeal stenosis, and keloids.