CRISPR/Cas9-guided knockout of eIF4E improves Wheat yellow mosaic virus resistance without yield penalty.
CRISPR/Cas9-guided knockout of eIF4E improves Wheat yellow mosaic virus resistance without yield penalty.
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DOI：
10.1111/pbi.14002
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发表时间：
2023-05
影响因子：
13.8
通讯作者：
Yang, Ping
中科院分区：
文献类型：
--
作者：
Kan, Jinhong;Cai, Yu;Cheng, Chunyuan;Chen, Shiqiang;Jiang, Congcong;He, Zhentian;Yang, Ping
关键词：
病毒是农业上最重要的植物病原体之一，占所有新出现的植物感染性疾病的47%（安德森等人，2004年）。基因组相对简单且仅编码少数蛋白质的病毒高度依赖于宿主因素（或易感性[S]因素）来完成其生命周期。S因子的修饰或突变可使感病性丧失，常导致隐性抗性。真核生物翻译起始因子4 E（eIF 4 E）是双子叶植物马铃薯Y病毒科马铃薯Y病毒属的一个S基因，编码一个与甲基化鸟嘌呤结合的帽结合蛋白，触发蛋白质翻译起始复合物的组装。eIF 4 E已成为使用CRISPR/Cas9（成簇的规则间隔短回文重复序列/CRISPR相关蛋白9）基因组编辑技术进行病毒抗性工程的主要靶标。敲除eIF 4 E显示在双子叶植物如甜瓜中产生抗性（Pechar等人，2022）和单子叶植物如大麦（Hoffie等，2021年）。小麦黄花叶病（Wheat yellow mosaic virus，WYMV）或中国小麦花叶病毒（ChineseWheat mosaic virus，CWMV）在东亚地区和小麦梭条花叶病（Wheat spindle streak mosaic virus，WSSMV）在欧洲和北美地区引起，严重影响六倍体小麦的产量。WYMV和WSSMV属于Potyviridae科的Bymovirus属，并通过土传的Plasmodiophorid Polymyxa graminis传播。在二倍体大麦中，大麦黄花叶病毒（BaYMV）和大麦轻度花叶病毒（BaMMV）引起黄花叶病，并且大多数抗性基因座由隐性等位基因表示（Jiang et al.，2020年）。相比之下，在小麦中已经定位了13个抗性基因和/或数量性状位点（QTL），并且全部以显性方式遗传。然而，迄今为止，尚未在小麦中通过图位克隆分离出针对病毒的抗性基因。本研究报告了CRISPR/Cas9指导的大麦易感因子基因HveIF 4 E的小麦正向同源基因组编辑。在大麦中，HveIF 4 E中的天然多态性是几种抗性等位基因的致病因子（Jiang et al.，2020），并且HveIF 4 E编辑的植物也显示出BaMMV抗性（Hoffie等人，2021年）。通过与小麦参考基因组序列的BLAST比对，鉴定出3个TaeIF 4 E同源基因
Viruses, one of the most agriculturally important groups of plant pathogens, account for 47% of all emerging plant infectious diseases (Anderson et al., 2004). Viruses with relatively simple genomes and encoding only a few proteins are highly dependent on host factors (or susceptibility [S] factors) to complete their lifecycles. The modification or mutation of S factors might cause the loss of susceptibility, often resulting in recessive resistance. The eukaryotic translation initiation factor 4 E (eIF4E) is a wellcharacterized S gene for many potyviruses of the family Potyviridae in dicots, which encodes a cap-binding protein that binds to methylated guanine triggering assembly of the protein translation initiation complex. The eIF4E has become a major target for engineering viral resistance using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) genome-editing technology. Knocking out eIF4E was shown to result in resistance in dicots such as melon (Pechar et al., 2022) and monocots such as barley (Hoffie et al., 2021). Wheat yellow mosaic disease caused by Wheat yellow mosaic virus (WYMV) or Chinese wheat mosaic virus (CWMV) in East Asia, and Wheat spindle streak mosaic virus (WSSMV) in Europe and North America, results in severe yield losses in hexaploid wheat. WYMV and WSSMV belong to the genus Bymovirus of the family Potyviridae and are transmitted by the soil-borne plasmodiophorid Polymyxa graminis. In diploid barley, the bymoviruses Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) cause yellow mosaic disease, and most resistance loci are represented by recessive alleles (Jiang et al., 2020). By contrast, 13 resistance genes and/or quantitative trait loci (QTL) have been mapped in wheat, and all are inherited in a dominant manner. However, to date, no resistance genes against viruses have been isolated by map-based cloning in wheat.This study reported CRISPR/Cas9-guided genome editing of wheat orthologous of the barley susceptibility factor gene HveIF4E. In barley, natural polymorphisms in HveIF4E are the causal agent of several resistance alleles (Jiang et al., 2020), and HveIF4E-edited plants also showed BaMMV resistance (Hoffie et al., 2021). Three TaeIF4E homoeologous genes were identified by BLAST against the reference genome sequence of wheat
影响因子：
7.2
作者：
Gauffier, Camille;Lebaron, Caroline;Gallois, Jean-Luc
通讯作者：
Gallois, Jean-Luc
影响因子：
13.8
作者：
Pechar, Giuliano S.;Donaire, Livia;Gosalvez, Blanca;Garcia-Almodovar, Carlos;Amelia Sanchez-Pina, Maria;Truniger, Veronica;Aranda, Miguel A.
通讯作者：
Aranda, Miguel A.
影响因子：
--
作者：
Hoffie RE;Otto I;Perovic D;Budhagatapalli N;Habekuß A;Ordon F;Kumlehn J
通讯作者：
Kumlehn J