Radiation hybrid mapping of 304 novel microsatellites in the domestic cat genome
Radiation hybrid mapping of 304 novel microsatellites in the domestic cat genome
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DOI：
10.1159/000075762
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发表时间：
2003-01-01
影响因子：
1.7
通讯作者：
Murphy, WJ
中科院分区：
文献类型：
--
作者：
Menotti-Raymond, M;David, VA;Murphy, WJ
有效利用家猫作为遗传和传染病的动物模型，需要开发和实施包含微卫星和保守编码基因标记的高质量基因图谱。先前的猫科动物连锁和辐射杂交图谱在所有染色体上缺乏足够的微卫星覆盖，无法有效地利用全基因组扫描。本文报道了猫基因组中304个新的多态重复位点的分离和基因组定位。使用基于自动荧光Taq-Man的测定，在家猫辐射杂交面板中绘制了新的位点。加上这304颗微卫星，猫基因组的微卫星总数达到580颗，RH图谱上的基因座总数达到1126个。微卫星现在跨越每个常染色体，平均间隔大约为每5厘米器官一个多态性STR，每2.7兆碱基覆盖一个标记的全基因组。这些基因座现在提供了一种有用的工具，用于进行全基因组扫描，以识别与感兴趣的表型相关的基因，例如与遗传性疾病、毛色、模式和形态相关的基因。这些资源也可以扩展到其余36种猫科动物进行种群遗传和进化基因组分析。版权所有(C) 2003 S. Karger AG，巴塞尔
Effective utilization of the domestic cat as an animal model for hereditary and infectious disease requires the development and implementation of high quality gene maps incorporating microsatellites and conserved coding gene markers. Previous feline linkage and radiation hybrid maps have lacked sufficient microsatellite coverage on all chromosomes to make effective use of full genome scans. Here we report the isolation and genomic mapping of 304 novel polymorphic repeat loci in the feline genome. The new loci were mapped in the domestic cat radiation hybrid panel using an automated fluorescent Taq-Man based assay. The addition of these 304 microsatellites brings the total number of microsatellites mapped in the feline genome to 580, and the total number of loci placed onto the RH map to 1,126. Microsatellites now span every autosome with an average spacing of roughly one polymorphic STR every five centimorgans, and full genome coverage of one marker every 2.7 megabases. These loci now provide a useful tool for undertaking full-genome scans to identify genes associated with phenotypes of interest, such as those relating to hereditary disease, coat color, patterning and morphology. These resources can also be extended to the remaining 36 species of the cat family for population genetic and evolutionary genomic analyses. Copyright (C) 2003 S. Karger AG, Basel