An investigation of the clonality of human autoimmune thyroglobulin antibodies and their light chains.
An investigation of the clonality of human autoimmune thyroglobulin antibodies and their light chains.
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人类自身免疫甲状腺球蛋白抗体及其轻链克隆性的研究。
DOI：
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发表时间：
1981
影响因子：
4.6
通讯作者：
I. Roitt
中科院分区：
文献类型：
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作者：
L. Nye;L. D. De Carvalho;I. Roitt
用等电聚焦法检测了31例不同疾病患者血清中的甲状腺球蛋白抗体。只有一个给出了单克隆反应的谱型，其他30个给出了多克隆反应的谱型。在一些血清中有克隆优势的证据，每个血清给出不同的谱型。用亲和层析纯化的5种甲状腺球蛋白抗体制备了轻链。与正常免疫球蛋白制备的轻链相比，在光谱型上没有限制。
Thyroglobulin antibodies in the sera from 31 patients with a variety of disorders were studied by isoelectric focusing. Only one gave a spectrotype indicative of a monoclonal response, the other 30 giving spectrotypes characteristic of polyclonal responses. There was evidence of clonal dominance in some of the sera and each gave a different spectrotype. Light chains were prepared from five thyroglobulin antibodies purified by affinity chromatography. There was no restriction in the spectrotypes when compared with light chains prepared from normal immunoglobulin.