Coriandrum sativum and Lavandula angustifolia Essential Oils: Chemical Composition and Activity on Central Nervous System
Coriandrum sativum and Lavandula angustifolia Essential Oils: Chemical Composition and Activity on Central Nervous System
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DOI：
10.3390/ijms17121999
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发表时间：
2016-12-01
影响因子：
5.6
通讯作者：
De Feo, Vincenzo
中科院分区：
文献类型：
--
作者：
Caputo, Lucia;Souza, Luceia Fatima;De Feo, Vincenzo
本研究的目的是测定熏衣草的化学成分。和Coriandrum sativum L.精油的作用机制，评价其对SH-SY 5 Y人神经母细胞瘤细胞的细胞毒作用，研究其对腺苷酸环化酶1（ADCY 1）和细胞外信号调节激酶（ERK）表达的影响，并探讨其可能的神经电生理效应。采用水蒸气蒸馏法提取挥发油，用气相色谱和气相色谱-质谱联用技术对挥发油成分进行分析。angustifolia和C.大蒜中的主要成分为芳樟醇（分别为33.1%和67.8%）。将SH-SY 5 Y细胞与不同浓度的精油和芳樟醇一起孵育。分别使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑MTT和Western印迹分析细胞活力和对ADCY 1和ERK表达的影响。用多电极阵列（MEA）为基础的方法，在大鼠皮层神经元的原代培养中研究了细胞电生理的变化。精油和芳樟醇显示不同的细胞毒活性。芳樟醇抑制ADCY 1和ERK的表达。神经网络受到L. angustifolia和C.大蒜精油显示出对自发电活动的浓度依赖性抑制。
The aims of this study are to determine the chemical composition of Lavandula angustifolia Mill. and Coriandrum sativum L. essential oils, to evaluate their cytotoxic effects in SH-SY5Y human neuroblastoma cells, to investigate whether an alteration of adenylate cyclase 1 (ADCY1) and of extracellular signal-regulated kinase (ERK) expression can take part in the molecular mechanisms of the essential oils, and to study their possible neuronal electrophysiological effects. The essential oils were obtained by hydrodistillation, and studied by GC and GC-MS. In the oils from L. angustifolia and C. sativum, linalool was the main component (33.1% and 67.8%, respectively). SH-SY5Y cells were incubated with different concentrations of essential oils and of linalool. Cell viability and effects on ADCY1 and ERK expression were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT and Western blotting, respectively. Variation in cellular electrophysiology was studied in primary cultures of rat cortical neurons with a multi-electrode array (MEA)-based approach. The essential oils and linalool revealed different cytotoxic activities. Linalool inhibited ADCY1 and ERK expression. Neuronal networks subjected to L. angustifolia and C. sativum essential oils showed a concentration-dependent inhibition of spontaneous electrical activity.