Cell wall α1-3glucans induce the aggregation of germinating conidia of Aspergillus fumigatus
Cell wall α1-3glucans induce the aggregation of germinating conidia of Aspergillus fumigatus
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DOI：
10.1016/j.fgb.2010.04.006
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发表时间：
2010-08-01
影响因子：
3
通讯作者：
Latge, Jean-Paul
中科院分区：
文献类型：
--
作者：
Fontaine, Thierry;Beauvais, Anne;Latge, Jean-Paul
烟曲霉分生孢子的萌发可分为四个阶段：打破休眠、各向同性膨胀、细胞极性建立和芽管形成。分生孢子的膨胀与液体培养基中产生大的分生孢子团块的多细胞聚集有关。通过添加α 1- 3葡聚糖酶可以特异性地防止分生孢子聚集。Swageni分生孢子特异性地粘附于不溶性α 1- 3葡聚糖链。电子显微镜研究表明，细胞壁α 1- 3葡聚糖链在溶胀过程中暴露在细胞表面。这些结果表明，细胞壁α 1- 3葡聚糖在膨胀的分生孢子之间的聚集中起着重要作用。用α 1- 3葡聚糖涂覆的乳胶珠粒进行的实验表明，α 1- 3葡聚糖链在它们之间相互作用，而不需要任何其他细胞壁组分，这表明α 1- 3葡聚糖的生物物理性质是分生孢子聚集的唯一原因。(C)2010年爱思唯尔公司All rights reserved.
The germination of Aspergillus fumigatus conidia can be divided into four stages: breaking of dormancy, isotropic swelling, establishment of cell polarity, and formation of a germ tube. Swelling of conidia is associated in liquid medium with a multi-cellular aggregation that produced large clumps of conidia. Conidial aggregation can be specifically prevented by the addition of alpha 1-3glucanase. Swollen conidia specifically adhere to insoluble alpha 1-3glucan chains. Electron microscopy studies showed that cell wall alpha 1-3glucan chains became exposed at the cell surface during the swelling. These results demonstrate that cell wall alpha 1-3glucans play an essential role in the aggregation between swollen conidia. Experiments with alpha 1-3glucan coated latex beads show that alpha 1-3glucan chains interacted between them without the requirement of any other cell wall component suggesting that biophysical properties of alpha 1-3glucans are solely responsible for conidial aggregation. (C) 2010 Elsevier Inc. All rights reserved.