Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas.
Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas.
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DOI：
10.1016/j.ecoenv.2011.05.019
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发表时间：
2011-09
影响因子：
6.8
通讯作者：
A. Babczyńska;G. Wilczek;E. Szulińska;I. Franiel
中科院分区：
文献类型：
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作者：
A. Babczyńska;G. Wilczek;E. Szulińska;I. Franiel
蜘蛛栖息在后工业环境中，如废物堆或含矿地区，暴露在高浓度的金属中，这些金属积累在猎物体内，并沿沿着转移。因此，蜘蛛被迫发展金属中和策略。低分子多功能蛋白质：金属硫蛋白（MT），通常被假定为金属暴露的生物标志物，已知可结合金属，从而保护生物体免受其毒性作用。然而，这些蛋白质在蜘蛛中仍然没有得到很好的识别。本研究的目的是通过免疫检测方法，ELISA，评估金属硫蛋白的浓度，在成年女性的三个网络建筑蜘蛛种：（Araneidae），Agelena arthica（Agelenidae）和三角林蝇Linyphia triangularis（Linyphiidae），来自波兰南部三个不同污染地区：奥尔库什，含矿后工业区;卡托维兹-韦诺维茨：采矿后废物堆，皮利卡：农村，农村，农村。分析了金属硫蛋白的浓度与蜘蛛体内金属浓度的关系。研究结果表明，金属硫蛋白酶联免疫吸附试验（ELISA）技术是可靠的。对所得结果的分析表明，MTs水平具有很强的物种依赖性。MTs含量与蜘蛛体内重金属（主要是Zn和Pb）含量呈正相关，表明MTs蛋白在蜘蛛体内对重金属离子的中和和调节中起重要作用。同样的相关性表明，有可能考虑在蜘蛛MT作为金属接触和影响的生物标志物。然而，应考虑到物种特异性以及金属特性。
Spiders inhabiting post industrial environments, such as waste heaps or ore-bearing areas, are exposed to high concentrations of metals, accumulated in the body of their prey and transferred along food chains. Therefore spiders are pressed to develop metal-neutralization strategies. Low-molecular, multifunction proteins: metallothioneins (MTs), often postulated as biomarkers of metal exposure, are known to bind metals and thus protect organisms against their toxic effects. Yet the proteins are still not well recognized in spiders. The aim of this study was to assess, by immunodetection method, ELISA, the concentration of metallothioneins in adult females of three web building spider species: Araneus diadematus (Araneidae), Agelena labyrinthica (Agelenidae) and Linyphia triangularis (Linyphiidae) from three variously polluted areas in southern Poland: Olkusz, ore-bearing post industrial site; Katowice–WeŁnowiec: post metallurgic waste heap, Pilica: the reference, rural, area. The concentration of metallothioneins has been analyzed in relation to the metal concentration in spiders body. The study gives the evidence that metallothioneins are reliably detectable by means of ELISA technique. The analysis of results obtained shows a strong species-dependence of the MTs level. Positive correlations between MTs concentration and metal body burden (mainly Zn and Pb) were found. This suggests that the proteins play an important role in the neutralization and regulation of metal ions in spiders. The same correlation indicate the possibility to consider MTs in spiders as biomarkers of metal exposure and effects. However, the species specificity as well as metal characteristics should be taken under account.