Quantifying morphological features of actin cytoskeletal filaments in plant cells based on mathematical morphology
Quantifying morphological features of actin cytoskeletal filaments in plant cells based on mathematical morphology
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DOI：
10.1016/j.jtbi.2015.10.031
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发表时间：
2016-01-21
影响因子：
2
通讯作者：
Mano, Shoji
中科院分区：
文献类型：
--
作者：
Kimori, Yoshitaka;Hikino, Kazumi;Mano, Shoji
通过量化生物结构的形态特性，我们可以更好地评估复杂的形状并检测生物体内微妙的形态变化。本文提出了一种基于形态学图像处理的形状分析方法，并将其应用于拟南芥根毛细胞肌动蛋白细胞骨架丝的图像分析。在植物细胞中，肌动蛋白细胞骨架丝在各种细胞过程中发挥着关键作用，例如囊泡运输和细胞器运动。植物细胞中囊泡和细胞器的动力学依赖于肌动蛋白细胞骨架丝，调节细胞分裂和细胞增大。为了更好地了解肌动蛋白依赖性细胞器运动，我们尝试量化根毛缺陷 3 (rhd3) 突变体的根毛细胞中肌动蛋白丝的组织。 RHD3 参与肌动蛋白组织，据报道其缺陷会影响各种囊泡和细胞器的动力学。我们测量了野生型和突变植物中肌动蛋白丝的三个形状特征。一个特征（厚度）被描绘在灰度上；其他的（描述二维空间中细丝网络模式的复杂性）被描述为二进制特征。细胞骨架丝的形态表型在野生型和突变体之间明显不同。检测到突变体之间细丝形态的细微变化并进行统计量化。 (C) 2015 Elsevier Ltd. 保留所有权利。
By quantifying the morphological properties of biological structures, we can better evaluate complex shapes and detect subtle morphological changes in organisms. In this paper, we propose a shape analysis method based on morphological image processing, and apply it to image analysis of actin cytoskeletal filaments in root hair cells of Arabidopsis thaliana. In plant cells, the actin cytoskeletal filaments have critical roles in various cellular processes such as vesicle trafficking and organelle motility. The dynamics of vesicles and organelles in plant cells depend on actin cytoskeletal filaments, regulating cell division and cell enlargement. To better understand the actin-dependent organelle motility, we attempted to quantify the organization of actin filaments in the root hair cells of the root hair defective 3 (rhd3) mutant. RHD3 is involved in actin organization, and its defect has been reported to affect the dynamics of various vesicles and organelles. We measured three shape features of the actin filaments in wild-type and mutant plants. One feature (thickness) was depicted on a grayscale; the others (describing the complexity of the filament network patterns in two-dimensional space) were depicted as binary features. The morphological phenotypes of the cytoskeletal filaments clearly differed between wild-type and mutant. Subtle variations of filament morphology among the mutants were detected and statistically quantified. (C) 2015 Elsevier Ltd. All rights reserved.