Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation.
Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation.
复制标题
对HIV-VLP刺激的响应外周血单核细胞的基因表达谱。
DOI：
10.1186/1471-2105-9-s2-s5
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发表时间：
2008-03-26
影响因子：
3
通讯作者：
Buonaguro FM
中科院分区：
文献类型：
--
作者：
Buonaguro L;Monaco A;Aricò E;Wang E;Tornesello ML;Lewis GK;Marincola FM;Buonaguro FM
杆状病毒表达的HIV-1 Pr 55 gag病毒样颗粒（HIV-VLP）诱导单核细胞来源的树突状细胞（MDDC）成熟和活化，并产生Th 1和Th 2特异性细胞因子。从正常健康供体获得并由HIV-VLP激活的MDDC的基因组转录谱的分析显示了参与表征MDDC激活和成熟的形态和功能变化的基因的调节。使用外周血单核细胞（PBMC）获得类似的数据，无需进一步选择，显示了直接和“简化”实验程序的可行性。本文所述的结果表明，在离体产生的MDDC中由HIV-VLP诱导的成熟模式也可以在表达CD 14的新鲜来源的PBMC中观察到，可能鉴定出个体对免疫原应答的遗传预测因子。
Baculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1- and Th2-specific cytokines. The analysis of genomic transcriptional profile of MDDCs, obtained from normal healthy donors and activated by HIV-VLPs, show the modulation of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation. Similar data are obtained using peripheral blood mononuclear cells (PBMCs), without further selection, showing the feasibility of a direct and “simplified” experimental procedure. The results here described show that the maturation pattern induced by HIV-VLPs in ex vivo generated MDDCs, can be observed also in CD14-expressing freshly derived PBMCs, with the possible identification of genetic predictors of individual response to immunogens.