The Expression of MicroRNA-21 in Bone Marrow Fluid is an Indicator of Hematological Disorders and Mortality.
The Expression of MicroRNA-21 in Bone Marrow Fluid is an Indicator of Hematological Disorders and Mortality.
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DOI：
10.31557/apjcp.2020.21.10.2817
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发表时间：
2020-10-01
期刊：
影响因子：
--
通讯作者：
Feng YH
中科院分区：
文献类型：
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作者：
Chang WT;Huang TL;Chen ZC;Feng YH
骨髓液（BMF）由各种成分组成，为细胞分化和重塑建立微环境。microRNA-21（miR-21）水平最近已成为不同疾病的新生物标志物。然而，传统的RNU 6 B（U6），用作细胞内miRNA的参考，可能不适合循环miRNA的标准化。 我们测量了13名健康对照和37名血液病患者BMF中U6、掺入RNA和miR-21的水平，以研究U6或掺入RNA作为内源性参考的可靠性，并研究miR-21、血液病和死亡率之间的相关性。 值得注意的是，U6的水平证明了健康对照和患者的BMF的高变异性。相比之下，加标RNA的水平在两个队列中显示出显著更高的稳定性。与对照组相比，白血病患者BMF中miR-21的水平显著上调，但淋巴瘤患者无此现象。此外，使用21作为miR-21的截止值，它区分了血液系统疾病患者的死亡率。 总的来说，使用掺入的RNA作为参考，BMF中上调的miR-21水平可以是白血病诊断的指标和死亡率的预测因子。
Bone marrow fluid (BMF) consists of various components that establishes a microenvironment for cell differentiation and remodeling. MicroRNA-21 (miR-21) levels have recently emerged as novel biomarkers for different diseases. However, the conventional RNU6B (U6), used as the reference for intracellular miRNA, may not be appropriate for the normalization of circulating miRNAs. We measured the levels of U6, spiked-in RNA, and miR-21 in the BMF of 13 healthy controls and 37 patients with hematological disorders to investigate the reliability of either U6 or spike-in RNA as an endogenous reference and also to study the correlation between miR-21, hematological disorders and mortality. Notably, the levels of U6 demonstrated a high variability in BMF of healthy controls and patients. In contrast, the levels of spiked-in RNA displayed a significantly higher stability in both cohorts. Compared with controls, the levels of miR-21 were significantly upregulated in BMF of patients with leukemia but not lymphoma. Also, using 21 as the cut-off value of miR-21, it differentiated the mortality of patients with hematologic disorders. Collectively, using spiked-in RNA as a reference the upregulated miR-21 levels in BMF could be an indicator of the diagnosis of leukemia and a predictor of mortality.