Activation of the canonical Wnt/β-catenin pathway enhances monocyte adhesion to endothelial cells
Activation of the canonical Wnt/β-catenin pathway enhances monocyte adhesion to endothelial cells
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DOI：
10.1016/j.bbrc.2006.06.082
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发表时间：
2006-08-18
影响因子：
3.1
通讯作者：
Wilson, Colleen L.
中科院分区：
文献类型：
--
作者：
Lee, Dong Kun;Grantham, R. Nathan;Wilson, Colleen L.
单核细胞与血管内皮细胞的粘附是动脉粥样硬化形成的早期过程之一。为了开发预防或延缓动脉粥样硬化进展的策略，我们分析了Wnt/β-连环蛋白信号通路对单核细胞与各种人内皮细胞粘附的影响。在荧光显微镜下分析荧光素标记的单核细胞与各种人内皮细胞的粘附。与氯化钠不同，氯化锂以剂量依赖性方式增强单核细胞与内皮细胞的粘附。我们进一步证明，糖原合成酶激酶（GSK）-3 β或蛋白酶体抑制剂增强单核细胞-内皮细胞粘附。半定量逆转录聚合酶链反应（RT-PCR）结果表明，Wnt/β-catenin通路的激活并不改变粘附分子mRNA的表达水平。总之，经典的Wnt/β-连环蛋白途径增强单核细胞-内皮细胞粘附而不改变粘附分子的表达水平。(c)2006年爱思唯尔公司All rights reserved.
Monocyte adhesion to vascular endothelium has been reported to be one of the early processes in the development of atherosclerosis. In an attempt to develop strategies to prevent or delay atherosclerosis progression, we analyzed effects of the Wnt/beta-catenin signaling pathway on monocyte adhesion to various human endothelial cells. Adhesion of fluorescein-labeled monocytes to various human endothelial cells was analyzed under a fluorescent microscope. Unlike sodium chloride, lithium chloride enhanced monocyte adhesion to endothelial cells in a dose-dependent manner. We further demonstrated that inhibitors for glycogen synthase kinase (GSK)-3 beta or proteosome enhanced monocyte-endothelial cell adhesion. Results of semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR) indicated that activation of Wnt/beta-catenin pathway did not change expression levels of mRNA for adhesion molecules. In conclusion, the canonical Wnt/beta-catenin pathway enhanced monocyte-endothelial cell adhesion without changing expression levels of adhesion molecules. (c) 2006 Elsevier Inc. All rights reserved.