Transfer of secretory proteins from the endoplasmic reticulum to the Golgi apparatus: discrimination between homologous and heterologous transfer in intact heterokaryons.
Transfer of secretory proteins from the endoplasmic reticulum to the Golgi apparatus: discrimination between homologous and heterologous transfer in intact heterokaryons.
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分泌蛋白从内质网转移到高尔基体：完整异核体中同源和异源转移的区别。
DOI：
10.1073/pnas.87.20.8175
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发表时间：
1990
影响因子：
11.1
通讯作者：
Singer,SJ
中科院分区：
文献类型：
--
作者：
Valtersson,C;Dutton,AH;Singer,SJ
为了检查分泌蛋白从内质网原位转移到高尔基体的各个方面，在 Hep G2 人肝癌细胞和 WI-38 人成纤维细胞之间形成异核体。细胞在融合前用放线菌酮进行适当处理，从而清空它们各自的分泌蛋白、Hep G2 细胞的血清白蛋白和 WI-38 细胞的前胶原 I。融合完成后，洗掉放线菌酮，恢复蛋白质合成，并通过免疫荧光显微镜观察每个异核内两个分离的高尔基体中血清白蛋白和前胶原I的重现率。发现血清白蛋白总是首先出现在由异核体的 Hep G2 一半贡献的高尔基体中，而原胶原 I 总是首先出现在 WI-38 一半的高尔基体中。这些结果表明，内质网到高尔基体的原位转移不仅仅是一个随机过程，而且要么受到空间限制，要么表现出细胞类型特异性，或者两者兼而有之。
To examine aspects of the transfer of secretory proteins from the endoplasmic reticulum to the Golgi apparatus in situ, heterokaryons were formed between Hep G2 human hepatoma cells and WI-38 human fibroblasts. The cells were appropriately treated with cycloheximide before fusion, which emptied them of their respective secretory proteins, serum albumin for the Hep G2 cells and procollagen I for the WI-38 cells. After fusion was complete, the cycloheximide was washed out, protein synthesis was resumed, and the rates of reappearance of serum albumin and procollagen I in the two separated Golgi apparatuses within each heterokaryon were followed by immunofluorescence microscopy. Serum albumin was found to always reappear first in the Golgi apparatus contributed by the Hep G2 half of the heterokaryon, and procollagen I in the Golgi apparatus of the WI-38 half. These results suggest that the endoplasmic reticulum-to-Golgi apparatus transfer in situ is not simply a stochastic process but is either spatially restricted or exhibits cell-type specificity or both.