Transmembrane Domain Peptide/Peptide Nucleic Acid Hybrid as a Model of a SNARE Protein in Vesicle Fusion

Transmembrane Domain Peptide/Peptide Nucleic Acid Hybrid as a Model of a SNARE Protein in Vesicle Fusion
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DOI:
10.1002/anie.201101951
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发表时间:
2011-01-01
影响因子:
16.6
通讯作者:
Diederichsen, Ulf
Diederichsen, Ulf
中科院分区:
化学1区
文献类型:
--
作者:
Lygina, Antonina S.;Meyenberg, Karsten;Diederichsen, Ulf

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所有工业溶剂在使用前均经过蒸馏。分析级和 HPLC 级溶剂按原样使用。无水DMF、乙醚、THF和DCM得自Aldrich、Fluka或Acros。用于肽合成的 N-甲基-2-吡咯烷酮 (NMP) 购自 Carl Roth GmbH,由 GS 控制,无需额外纯化即可使用。哌啶从 Riedel de Haen 获得并按提供的形式使用。乙腈和甲醇均为 HPLC 级。
All technical solvents were distilled prior to use. The solvents of analytical and HPLC grade were used as supplied. Anhydrous DMF, diethyl ether, THF and DCM were obtained from Aldrich, Fluka or Acros. N-Methyl-2-pyrrolidon (NMP) for peptide synthesis was obtained from Carl Roth GmbH, controlled by GS and used without additional purification. Piperidine was obtained from Riedel de Haen and used as supplied. Acetonitrile and methanol were of HPLC grade.