Cytoskeletal coordination during neuroinal migration
Cytoskeletal coordination during neuroinal migration
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DOI：
10.1073/pnas.0506008102
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发表时间：
2005-09-20
影响因子：
11.1
通讯作者：
McConnell, SK
中科院分区：
文献类型：
--
作者：
Schaar, BT;McConnell, SK
人类和小鼠遗传学的发现已经确定了细胞骨架和信号蛋白，这些蛋白对发育中的大脑中的神经元迁移至关重要。为了给这些研究提供一个有意义的背景，我们采取了一种无偏见的方法，对通过三维基质迁移的神经元进行相关电子显微镜观察，并描述了当迁移的神经元启动细胞核的跳跃式向前移动时发生的细胞骨架事件。在核移位之前，在近端引导突起形成细胞质扩张。在跳跃运动中，细胞核移位到这些扩张的地方。延时成像和药理学扰动表明，核运动需要微管、肌球蛋白II和细胞黏附之间的逐步或分级相互作用。我们假设，在神经元迁移过程中，这些相互作用耦合导致核移位的过程延伸。
Discoveries from human and mouse genetics have identified cytoskeletal and signaling proteins that are essential for neuronal migration in the developing brain. To provide a meaningful context for these studies, we took an unbiased approach of correlative electron microscopy of neurons migrating through a three-dimensional matrix, and characterized the cytoskeletal events that occur as migrating neurons initiate saltatory forward movements of the cell nucleus. The formation of a cytoplasmic dilation in the proximal leading process precedes nuclear translocation. Cell nuclei translocate into these dilations in saltatory movements. Time-lapse imaging and pharmacological perturbation suggest that nucleokinesis requires stepwise or hierarchical interactions between microtubules, myosin II, and cell adhesion. We hypothesize that these interactions couple leading process extension to nuclear translocation during neuronal migration.