Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli

Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli
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DOI:
10.1128/jb.179.2.538-540.1997
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发表时间:
1997-01-01
影响因子:
3.2
通讯作者:
Meyer, M
Meyer, M
中科院分区:
生物学3区
文献类型:
--
作者:
Dehio, C;Meyer, M

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本文报道了从大肠杆菌到汉赛巴尔通体的接合质粒转移。产生了携带表达各种抗性标记的不亲和性组P(IncP)和Q(IncQ)的质粒的转移接合物。通过接合在自杀质粒上递送的Tn5转座子显示转座插入随机染色体位点。
The first demonstration of conjugal plasmid transfer from Escherichia coli to Bartonella henselae is reported. Transconjugants bearing plasmids of incompatibility groups P (IncP) and Q (IncQ), expressing various resistance markers, were generated. Tn5 transposons delivered on suicide plasmids by conjugation showed transpositional insertion into random chromosomal sites.