Biological properties of syncytium-forming ("foamy") viruses.
Biological properties of syncytium-forming ("foamy") viruses.
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DOI：
10.1016/0042-6822(72)90557-0
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发表时间：
1972-03
期刊：
影响因子：
3.7
通讯作者：
W. Parks;G. Todaro
中科院分区：
文献类型：
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作者：
W. Parks;G. Todaro
来自牛、猫和猿种属的合胞体形成病毒在多种种属的分裂细胞培养物中生长至最高滴度，并具有相似的细胞病变效应。免疫荧光法检测到核内和胞浆抗原。建立了8天空斑试验，测定了猴泡沫病毒3型的生长周期;有一个相对较长的潜伏期为26小时。即使在最佳条件下，病毒产量也很低，每24小时不超过10个噬斑形成单位/细胞。与其他具有RNA指导的DNA聚合酶的病毒一样，低浓度可显著抑制感染性猿猴“泡沫”3型的形成（10 μg/ml）胸苷类似物BrdU;类似地，“泡沫状”病毒感染性也对紫外线灭活具有极强的抵抗力，这表明病毒的另一个共同的生物学特征是具有逆转录酶。
Syncytium-forming viruses from bovine, feline, and simian species grew to highest titers in dividing cell cultures from a variety of species and had similar cytopathic effects. Both intranuclear and cytoplasmic antigens were detected by immunofluo-rescence. An 8-day plaque assay was developed, and the growth cycle of simian “foamy” virus type 3 was determined; there was a relatively prolonged latent period of 26 hr. Even with optimal conditions, virus yields were low and did not exceed 10 plaque-forming units/cell per 24 hr. Like other viruses with an RNA-directed DNA polymerase, the formation of infectious simian “foamy” type 3 was markedly inhibited by low concentrations (10 μg/ml) of the thymidine analog, BrdU; similarly “foamy” virus infectivity was also extremely resistant to inactivation by ultraviolet light, suggesting another shared biological feature of viruses with a reverse transcriptase.