A Network Pharmacology Approach to Uncover the Mechanisms of Shen-Qi-Di-Huang Decoction against Diabetic Nephropathy
A Network Pharmacology Approach to Uncover the Mechanisms of Shen-Qi-Di-Huang Decoction against Diabetic Nephropathy
复制标题
网络药理学方法揭示参芪地黄汤抗糖尿病肾病的作用机制
DOI：
10.1155/2018/7043402
复制
发表时间：
2018-01-01
影响因子：
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通讯作者：
Tong, Xiaolin
中科院分区：
文献类型：
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作者：
Di, Sha;Han, Lin;Tong, Xiaolin
参芪地黄汤是我国著名的中药方剂，在糖尿病肾病（DN）的治疗中有着广泛的应用。然而，SQDHD的药理学机制尚未完全阐明。首先，我们进行了全面的文献检索，以确定SQDHD的活性成分，确定其相应的目标，并从几个数据库中获得已知的DN目标。然后构建蛋白质-蛋白质相互作用网络，以探索SQDHD靶标与已知治疗DN的靶标之间的复杂关系。通过对网络中各节点的拓扑特征筛选，得到了400个SQDHD的主要目标。从大卫获得的途径富集分析结果显示，重要的生物过程和途径包括氧化应激、对葡萄糖的反应、血压调节、细胞增殖调节、甜菜碱介导的信号通路和凋亡信号通路。更有趣的是，SQDHD的五个关键靶点，即AKT 1，AR，CTNNB 1，EGFR和ESR 1，在上述生物过程和途径的调节中具有重要意义。本研究从整体角度部分验证和预测了SQDHD治疗DN的药理和分子机制。这为进一步的实验研究奠定了基础，也拓展了SQDHD在临床上的合理应用。
Shen-Qi-Di-Huang decoction (SQDHD), a well-known herbal formula from China, has been widely used in the treatment of diabetic nephropathy (DN). However, the pharmacological mechanisms of SQDHD have not been entirely elucidated. At first, we conducted a comprehensive literature search to identify the active constituents of SQDHD, determined their corresponding targets, and obtained known DN targets from several databases. A protein-protein interaction network was then built to explore the complex relations between SQDHD targets and those known to treat DN. Following the topological feature screening of each node in the network, 400 major targets of SQDHD were obtained. The pathway enrichment analysis results acquired from DAVID showed that the significant bioprocesses and pathways include oxidative stress, response to glucose, regulation of blood pressure, regulation of cell proliferation, cytokine-mediated signaling pathway, and the apoptotic signaling pathway. More interestingly, five key targets of SQDHD, named AKT1, AR, CTNNB1, EGFR, and ESR1, were significant in the regulation of the above bioprocesses and pathways. This study partially verified and predicted the pharmacological and molecular mechanisms of SQDHD on DN from a holistic perspective. This has laid the foundation for further experimental research and has expanded the rational application of SQDHD in clinical practice.