MHC class II molecules, cathepsins, and La/SSB proteins in lacrimal acinar cell endomembranes.
MHC class II molecules, cathepsins, and La/SSB proteins in lacrimal acinar cell endomembranes.
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泪腺腺泡细胞内膜中的 MHC II 类分子、组织蛋白酶和 La/SSB 蛋白。
DOI：
10.1152/ajpcell.1999.277.5.c994
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Mircheff,AK
中科院分区：
文献类型：
--
作者：
Yang,T;Zeng,H;Zhang,J;Okamoto,CT;Warren,DW;Wood,RL;Bachmann,M;Mircheff,AK
̈综合征是一种慢性自身免疫性疾病，影响泪腺和其他上皮细胞。有人认为泪腺的腺泡细胞在开始表达主要组织相容性复合体(MHC)II类分子时，会引起局部自身免疫反应，从而导致̈综合征。我们使用等密度离心法和相分离来解决参与基底膜和内膜系统之间运输的隔室，以检验MHC II类分子进入含有潜在自身抗原(即La/SSB)和能够蛋白水解性处理自身抗原的酶(即组织蛋白B和D)的隔室的假设。一系列被鉴定为分泌囊泡膜、溶酶体前和反高尔基网络微域的隔室参与运输到基侧膜、分泌囊泡和溶酶体前分子，都是MHC II类、La/SSB和溶酶体前分子的显著基因座。和组织蛋白酶B和D。这些观察结果支持泪腺腺泡细胞被诱导表达MHC-II类分子，其功能是作为自身抗原处理和提呈细胞。
Sjögren's syndrome is a chronic autoimmune disease affecting the lacrimal glands and other epithelia. It has been suggested that acinar cells of the lacrimal glands provoke local autoimmune responses, leading to Sjögren's syndrome when they begin expressing major histocompatibility complex (MHC) class II molecules. We used isopycnic centrifugation and phase partitioning to resolve compartments that participate in traffic between the basolateral membranes and the endomembrane system to test the hypothesis that MHC class II molecules enter compartments that contain potential autoantigens, i.e., La/SSB, and enzymes capable of proteolytically processing autoantigen, i.e., cathepsins B and D. A series of compartments identified as secretory vesicle membranes, prelysosomes, and microdomains of the trans-Golgi network involved in traffic to the basolateral membrane, to the secretory vesicles, and to the prelysosomes were all prominent loci of MHC class II molecules, La/SSB, and cathepsins B and D. These observations support the thesis that lacrimal gland acinar cells that have been induced to express MHC class II molecules function as autoantigen processing and presenting cells.