Formation of lipophilic drug-loaded human serum albumin nanofibers with the aid of glutathione
Formation of lipophilic drug-loaded human serum albumin nanofibers with the aid of glutathione
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DOI：
10.1016/j.cej.2016.12.121
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发表时间：
2017-04
影响因子：
15.1
通讯作者：
Arjun Prasad Tiwari;Mahesh Kumar Joshi;Bikendra Maharjan;Joshua Lee;C. Park;Cheol-Sang Kim
中科院分区：
文献类型：
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作者：
Arjun Prasad Tiwari;Mahesh Kumar Joshi;Bikendra Maharjan;Joshua Lee;C. Park;Cheol-Sang Kim
我们首次报道了一种制备疏水载药人血清白蛋白纳米纤维的有效方法。纳米纤维的成功形成与谷胱甘肽（GSH）浓度、溶液温度和加热时间密切相关。利用电子显微镜、傅里叶变换红外光谱（FTIR）和x射线粉末衍射（XRD）对制备的纳米纤维进行了表征。电子显微图显示，纳米纤维具有圆柱形形态，直径70 ~ 120nm，长度可达几微米，表面光滑。GSH有助于HSA在高温（80-85℃）下快速展开，从而导致与紫杉醇的强相互作用，导致从纳米颗粒到纳米纤维的形态转变。此外，还讨论了纳米纤维形成的可能机制。
We report an efficient approach for the fabrication of hydrophobic drug-loaded human serum albumin nanofibers for the first time. The successful formation of nanofibers was found closely related to the glutathione (GSH) concentration, solution temperature, and heating time. As-fabricated nanofibers were characterized by electron microscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD). The electron micrographs show that nanofibers have cylindrical morphology and diameters of 70–120 nm and lengths of up to few micrometers with a smooth surface. GSH was found to contribute to the quicker unfolding of the HSA under high temperature (80–85 °C) which resulted in the strong interaction with paclitaxel, leading to a morphological transformation from nanoparticles to nanofibers. In addition, the possible mechanisms of nanofiber formation have been discussed.