A type-specific blocking ELISA for the detection of infectious bronchitis virus antibody
A type-specific blocking ELISA for the detection of infectious bronchitis virus antibody
复制标题
DOI：
10.1016/j.jviromet.2010.12.016
复制
发表时间：
2011-04-01
影响因子：
3.1
通讯作者：
Cheng, Ivan-Chen
中科院分区：
文献类型：
--
作者：
Chen, Hui-Wen;Wang, Ching-Ho;Cheng, Ivan-Chen
传染性支气管炎病毒(IBV)感染一直是全球养禽业的主要威胁。目前市面上已有的检测IBV群特异性抗体的试剂盒，为了了解现场感染情况，建立了一种针对本地IBV的单抗阻断ELISA(b-ELISA)。所选单抗对台湾IBV毒株具有特异性，但与疫苗株H120无交叉反应。以血凝抑制(HI)试验为标准，对390份现场标本进行了该单抗b-EL ISA的临界值、灵敏度和特异度的评价。用一组鸡的高免血清验证了检测的类型特异性。结果表明，该方法具有较高的敏感性(98.0%)和特异性(97.2%)。B-酶联免疫吸附试验与血凝抑制试验结果的一致性有统计学意义(Kappa=0.95)，两种方法之间无显著差异(McNemar p=0.72)。B-酶联免疫吸附试验检测到台湾IBV血清型，但没有检测到三种非台湾IBV血清型，也没有检测到抗其他禽病原体的血清。这种b-ELISA提供了本地IBV毒株的类型特异性抗体检测。它有可能成为台湾地区IBV临床感染的一种快速、可靠的诊断方法。(C)2011爱思唯尔B.V.保留所有权利。
Infectious bronchitis virus (IBV) infection has been a major threat to the poultry industry worldwide. Current commercially available ELISA kits detect group-specific antibodies; however, to understand the status of field infection, a monoclonal antibody (mAb) blocking ELISA (b-ELISA) against local IBVs was developed. The selected mAb showed specificity to Taiwan IBV strains but had no cross-reactivity with the vaccine strain H120. Using the hemagglutination inhibition (HI) test as a standard, the cut-off value, sensitivity, and specificity of a b-ELISA using this mAb were evaluated in 390 field samples. The type-specificity of detection was validated using a panel of chicken hyperimmune sera. The results showed that the b-ELISA demonstrated high sensitivity (98.0%) and specificity (97.2%) of detection. The agreement between the results of the b-ELISA and the HI test was statistically significant (Kappa = 0.95), and there was no significant difference between these two methods (McNemar p = 0.72). The b-ELISA specifically detected Taiwan IBV serotypes but not three non-Taiwan IBV serotypes nor sera against other avian pathogens. This b-ELISA provides type-specific antibody detection of local IBV strains. It has the potential to serve as a rapid and reliable diagnostic method for IBV clinical infections in the field in Taiwan. (C) 2011 Elsevier B.V. All rights reserved.