Insulin-like growth factor 1 sustained-release collagen on urethral catheter prevents stricture after urethral injury in a rabbit model
Insulin-like growth factor 1 sustained-release collagen on urethral catheter prevents stricture after urethral injury in a rabbit model
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DOI：
10.1111/iju.13931
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发表时间：
2019-05-01
影响因子：
2.6
通讯作者：
Horiguchi, Akio
中科院分区：
文献类型：
--
作者：
Shinchi, Masayuki;Kushibiki, Toshihiro;Horiguchi, Akio
目的 评价胰岛素样生长因子1缓释胶原导尿管对兔尿道损伤后尿道狭窄的预防作用。方法制作含胰岛素样生长因子1的胶原蛋白或仅含胶原蛋白的导尿管，将19只雄性日本大白兔根据电凝损伤尿道后立即插入的导尿管类型分为三组。第 1 组（n = 7）插入了一根涂有胰岛素样生长因子 1 浸渍胶原蛋白的导管；第 2 组（n = 7）插入仅涂有胶原蛋白的导管；第 3 组 (n = 5) 插入了未涂层的导管。总共14天后，对受伤的尿道进行尿道造影和尿道镜评估，并进行组织学检查。结果 尿道造影显示，第1组损伤尿道尿道管腔直径与正常尿道尿道管腔直径之比最大（P < 0.0001）。此外，第 1 组中的 7 只兔子中有 5 只 (71.4%) 的尿道腔足够大，可以通过尿道镜，这一比例大于第 2 组 (57.1%) 和第 3 组 (20%) 中的相应比例。组织学分析显示，第1组未覆盖再生尿道上皮的损伤面积往往小于其他两组，但平均差异不显着（P = 0.19）。结论 胰岛素样生长因子1缓释胶原导尿管可显着促进伤口愈合，预防尿道损伤后尿道狭窄。
Objectives To evaluate the preventive effect of an insulin-like growth factor 1 sustained-release collagen urethral catheter on urethral stricture after urethral injury in a rabbit model. Methods We made urethral catheters coated either with insulin-like growth factor 1 impregnated collagen or with only collagen, and we divided 19 male Japanese white rabbits into three groups according to the kind of catheter inserted immediately after the rabbit's urethra was injured by electrocoagulation. Group 1 (n = 7) had a catheter coated with insulin-like growth factor 1 impregnated collagen inserted; group 2 (n = 7) had a catheter coated with only collagen inserted; and group 3 (n = 5) had an uncoated catheter inserted. A total of 14 days later, the injured urethras were evaluated by urethrography and urethroscopy, and were also histologically examined. Results Urethrography showed that the ratio of the urethral lumen diameter in injured urethra to that in normal urethra was the largest in group 1 (P < 0.0001). In addition, five of the seven rabbits in group 1 (71.4%) had a urethral lumen large enough for passage of a urethroscope, a fraction larger than the corresponding fractions in groups 2 (57.1%) and 3 (20%). On histological analysis, the injured area not covered with regenerated urethral epithelium tended to be smaller in group 1 than the other two groups, but the mean difference was not significant (P = 0.19). Conclusions An insulin-like growth factor 1 sustained-release collagen urethral catheter significantly improves wound healing and prevents urethral stricture after urethral injury.