Microfluidic inverse phase ELISA via manipulation of magnetic beads
Microfluidic inverse phase ELISA via manipulation of magnetic beads
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DOI：
10.1007/s10404-010-0692-2
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发表时间：
2011-03-01
影响因子：
2.8
通讯作者：
Faghri, Mohammad
中科院分区：
文献类型：
--
作者：
Chen, Hong;Abolmatty, Assem;Faghri, Mohammad
我们报告了一种在微流控平台上进行免疫诊断的新技术。该平台不是针对固定固相处理流体试剂，而是通过流体试剂的固定塞操纵分析物涂覆的磁珠以检测抗原分析物。这些隔离但可接近的塞子通过毛细管力预先封装在微通道中。我们称这个平台为微流控反相酶联免疫吸附测定（μ IPELISA）。μ IPELISA在微流控免疫分析家族中具有独特的优势。特别地，它避免了在测定期间泵送和阀控流体试剂，从而导致芯片上实验室的形式，其不需要用于流体致动和控制的仪器。我们使用μ IPELISA检测从E. coliO 157：H7的PCR检测能力，并与微孔板ELISA检测能力进行比较。对于0.259 ng mu l（-1）地高辛标记的扩增子，mu IPELISA与微孔板ELISA一样敏感。此外，我们同时进行μ IPELISA在两个平行的微通道。
We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are pre-encapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (mu IPELISA). mu IPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use mu IPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng mu l(-1) of digoxigenin-labeled amplicon, mu IPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct mu IPELISA in two parallel microchannels.