Distinct mechanisms triggering glial differentiation in Drosophila thoracic and abdominal Neuroblasts 6-4
Distinct mechanisms triggering glial differentiation in Drosophila thoracic and abdominal Neuroblasts 6-4
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DOI：
10.1006/dbio.2000.9727
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发表时间：
2000-06-15
影响因子：
2.7
通讯作者：
Oda, H
中科院分区：
文献类型：
--
作者：
Akiyama-Oda, Y;Hotta, Y;Oda, H
在果蝇中枢神经系统发育过程中，神经母细胞分裂后，神经元和神经胶质细胞以固定模式产生。胸神经母细胞 6-4 (NB6-4T) 的第一次细胞分裂是不对称的，产生神经胶质前体细胞和神经元前体细胞。相反，腹部 NB6-4 (NB6-4A) 对称分裂产生两个神经胶质细胞。为了了解细胞分裂和胶质神经元细胞命运决定之间的关系，我们检查并比较了已知细胞分裂突变对 NB6-4T 和 NB6-4A 谱系的影响。根据对神经胶质细胞命运决定和早期神经胶质细胞分化基因表达的观察，当细胞周期进程和胞质分裂都被基因抑制时，神经胶质细胞分化的开始发生在NB6-4A中，而不是NB6-4T中。另一方面，当胞质分裂被完整的细胞周期进程阻断时，两个谱系中的神经胶质细胞分化都开始。这些结果表明，NB6-4T（而非 NB6-4A）需要细胞周期进程才能获得神经胶质细胞的命运，这表明不同的机制触发不同谱系的神经胶质细胞分化。 (C) 2000 年学术出版社。
Neurons and glia are produced in stereotyped patterns after neuroblast cell division during development of the Drosophila central nervous system. The first cell division of thoracic neuroblast 6-4 (NB6-4T) is asymmetric, giving rise to a glial precursor cell and a neuronal precursor cell. In contrast, abdominal NB6-4 (NB6-4A) divides symmetrically to produce two glial cells. To understand the relationship between cell division and glia-neuron cell fate determination, we examined and compared the effects of known cell division mutations on the NB6-4T and NB6-4A lineages. Based on observation of expression of glial fate determination and early glial differentiation genes, the onset of glial differentiation occurred in NB6-4A but not in NB6-4T when both cell cycle progression and cytokinesis were genetically arrested. On the other hand, glial differentiation started in both lineages when cytokinesis was blocked with intact cell cycle progression. These results showed that NB6-4T, but not NB6-4A, requires cell cycle progression for acquisition of glial fate, suggesting that distinct mechanisms trigger glial differentiation in the different lineages. (C) 2000 Academic Press.