Comparison of pulse sequences for R1-based electron paramagnetic resonance oxygen imaging
Comparison of pulse sequences for R1-based electron paramagnetic resonance oxygen imaging
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DOI：
10.1016/j.jmr.2015.02.012
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发表时间：
2015-05-01
影响因子：
2.2
通讯作者：
Halpern, Howard J.
中科院分区：
文献类型：
--
作者：
Epel, Boris;Halpern, Howard J.
电子顺磁共振（EPR）自旋晶格弛豫（SLR）氧成像已被证明是一个不可或缺的工具，评估活动物的氧分压。结合高精度和空间分辨率，EPR氧图像显示出显着的氧准确性。开发更有效的方法来获得SLR率具有重要的实用性、生物学和医学意义。在这项工作中，我们比较了不同的脉冲EPR成像协议和脉冲序列，以建立每种方法的优点和适用范围。使用含有旋转探针的体模进行测试，其中氧浓度与体内血氧测定相关。我们已经发现，对于小动物大小的对象，反演恢复序列结合滤波反投影重建方法提供了最好的准确度和精度。对于大型动物，其中大的射频能量沉积可能是至关重要的，自由感应衰减和三脉冲刺激回波序列可能会发现更好的实际用途。(C)2015 Elsevier Inc. All rights reserved.
Electron paramagnetic resonance (EPR) spin-lattice relaxation (SLR) oxygen imaging has proven to be an indispensable tool for assessing oxygen partial pressure in live animals. EPR oxygen images show remarkable oxygen accuracy when combined with high precision and spatial resolution. Developing more effective means for obtaining SLR rates is of great practical, biological and medical importance. In this work we compared different pulse EPR imaging protocols and pulse sequences to establish advantages and areas of applicability for each method. Tests were performed using phantoms containing spin probes with oxygen concentrations relevant to in vivo oxymetry. We have found that for small animal size objects the inversion recovery sequence combined with the filtered backprojection reconstruction method delivers the best accuracy and precision. For large animals, in which large radio frequency energy deposition might be critical, free induction decay and three pulse stimulated echo sequences might find better practical usage. (C) 2015 Elsevier Inc. All rights reserved.