Automated Determination of 13-Cis-and All-Trans-Retinoic Acid, Their 4-Oxo-Metabolites and Retinol in Plasma, Amniotic Fluid and Embryo by Reversed-Phase High-Performance Liquid Chromatography with a Precolumn Switching Technique
Automated Determination of 13-Cis-and All-Trans-Retinoic Acid, Their 4-Oxo-Metabolites and Retinol in Plasma, Amniotic Fluid and Embryo by Reversed-Phase High-Performance Liquid Chromatography with a Precolumn Switching Technique
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采用柱前切换技术的反相高效液相色谱自动测定血浆、羊水和胚胎中的 13-顺式和全反式视黄酸、其 4-氧代谢物和视黄醇
DOI：
10.1080/01483918808069040
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发表时间：
1988
影响因子：
1.3
通讯作者：
H. Nau
中科院分区：
文献类型：
--
作者：
J. Kraft;Ch. Echoff;W. Kuhnz;B. Löfberg;H. Nau
本文介绍了一种全自动柱前切换反相高效液相色谱（HPLC）法，可定量测定血浆、羊水和组织匀浆中的13-顺式维甲酸和全反式维甲酸及其4-氧代代谢产物和视黄醇。二元梯度系统允许在15分钟内基线分离类维生素A。样品制备保持简单，以最大限度地减少不稳定物质的降解和异构化，仅需要添加异丙醇，在液氮中冷冻和离心。总回收率是定量的，允许外部标准化。在4个数量级的浓度范围内，小鼠血浆、羊水和人血清白蛋白溶液（r < 0.99）和小鼠胚胎匀浆（r < 0.98）的校准曲线呈线性。在0.1ml或0.1g的样品量下，检测限为2ng/ml或2g。方法的变异系数小于5%（w/w）。
Abstract A fully automated reversed-phase high-performance liquid chromatography (HPLC) procedure with precolumn switching is presented which allows quantitative assay of 13-cis-retinoic acid and all-trans-retinoic acid, their 4-oxo-metabolites and retinol in plasma, amniotic fluid and tissue homogenates. A binary gradient system allowed baseline separation of the retinoids within 15 minutes. Sample preparation was kept simple in order to minimize degradation and isomerization of the unstable substances and required only the addition of isopropanol, freezing in liquid nitrogen, and centrifugation. Overall recovery was quantitative allowing for external standardization. Calibration curves were linear in mouse plasma, amniotic fluid and human serum albumin solution (r < 0.99) and in mouse embryo homogenate (r < 0.98) over a concentration range of 4 orders of magnitude. The detection limit was 2 ng/ml or g with a sample size of only 0.1 ml or 0.1 g. Coefficients of variation of the assay were less than 5% (w...
小鼠中 13-顺式视黄酸（异维A酸）的低致畸性对应于器官发生过程中的低胚胎浓度：与全反式异构体相比。
影响因子：
3.8
作者：
Kraft,JC;Kochhar,DM;Scott,WJ;Nau,H
通讯作者：
Nau,H