The Kodak Ektachem 400 Analyzer evaluated for automated enzymic determination of plasma creatinine.
The Kodak Ektachem 400 Analyzer evaluated for automated enzymic determination of plasma creatinine.
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Kodak Ektachem 400 分析仪对血浆肌酐的自动酶测定进行了评估。
DOI：
10.1093/clinchem/29.7.1422
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发表时间：
1983
影响因子：
9.3
通讯作者：
J. Ladenson
中科院分区：
文献类型：
--
作者：
C. Smith;M. Landt;M. Steelman;J. Ladenson
我们已经评估了用于血浆肌酐分析的自动肌酸酐亚氨基水解酶程序，该程序用于柯达Ektachem 400分析仪。与动力学和终点（平衡）碱性苦味酸过程的相关性通常很好。酶促过程的优点是不受胆红素、头孢菌素和乙酰乙酸的影响，所有这些都会干扰碱性苦味酸过程。然而，其准确性在很大程度上取决于制造商提供的校准值。在-20℃下储存样品时，测定的肌酐变化不大。Ektachem方法比碱性苦克酸盐方法具有更大的线性范围（140 mg/L），但其缺点是在正常范围内精度不理想。肌酸酐亚氨基水解酶的特异性增强、易于操作和其他优点使其成为一种有吸引力的方法。
We have evaluated an automated creatinine iminohydrolase procedure for plasma creatinine analysis, as used in the Kodak Ektachem 400 Analyzer. The correlation with kinetic and end-point (equilibrium) alkaline picrate procedures is generally excellent. The enzymatic procedure has the advantage of being unaffected by bilirubin, cephalosporins, and acetoacetate, all of which interfere in alkaline picrate procedures. Its accuracy, however, greatly depends on calibration values provided by the manufacturer. Storage of samples at -20 degrees C produced little change in measured creatinine. The Ektachem procedure has a greater range of linearity (140 mg/L) than the alkaline picrate procedures, but it shares with them the disadvantages of suboptimal precision in the normal range. The increased specificity, ease of performance, and other advantages of this use of creatinine iminohydrolase make it an attractive procedure.