IDENTIFICATION OF A 2ND HOMOLOG OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN THAT IS EXPRESSED IN THE NERVOUS-SYSTEM AND SECRETORY-TISSUES OF DROSOPHILA
IDENTIFICATION OF A 2ND HOMOLOG OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN THAT IS EXPRESSED IN THE NERVOUS-SYSTEM AND SECRETORY-TISSUES OF DROSOPHILA
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DOI：
10.1073/pnas.92.15.7095
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发表时间：
1995-07-18
影响因子：
11.1
通讯作者：
TRIMBLE, WS
中科院分区：
文献类型：
--
作者：
BOULIANNE, GL;TRIMBLE, WS
N-乙基马来酰亚胺敏感性融合蛋白（NSF）是一种ATP酶，已知在细胞内膜转运事件中具有重要作用。最近，cDNA克隆编码果蝇同源的这种蛋白质，命名为dNSF，其特征在于，并发现在神经系统中表达。我们现在报告的NSF，称为dNSF-2在这个物种中的第二个同源物的鉴定和报告的证据表明，这种无处不在的和广泛使用的融合蛋白属于一个多基因家族。预测的dNSF-2的氨基酸序列与dNSF（以下称为dNSF-1）的同源性为84.5%，与中国仓鼠的NSF的同源性为59%，与酵母同源物SEC 18的同源性为38.5%。最高的相似性被发现在一个区域的dNSF-2包含两个ATP结合位点之一，这个区域是最相似的一个超家族的ATP酶的成员。dNSF-2定位于3号染色体上87 F12和88 A3带之间的区域，原位杂交技术揭示了在胚胎发生期间的神经系统和幼虫的几个成虫盘和分泌结构中的表达。dNSF-2表达的发育调节表明，分泌器的数量变化是重要的组织发生。
N-Ethylmaleimide-sensitive fusion protein (NSF) is an ATPase known to have an essential role in intracellular membrane transport events. Recently, cDNA clones encoding a Drosophila melanogaster homolog of this protein, named dNSF, were characterized and found to be expressed in the nervous system. We now report the identification of a second homolog of NSF, called dNSF-2 within this species and report evidence that this ubiquitous and widely utilized fusion protein belongs to a multigene family. The predicted amino acid sequence of dNSF-2 is 84.5% identical to dNSF (hereafter named dNSF-1), 59% identical to NSF from Chinese hamster, and 38.5% identical to the yeast homolog SEC18. The highest similarity was found in a region of dNSF-2 containing one of two ATP-binding sites; this region is most similar to members of a superfamily of ATPases. dNSF-2 is localized to a region between bands 87F12 and 88A3 on chromosome 3, and ill situ hybridization techniques revealed expression in the nervous system during embryogenesis and in several imaginal discs and secretory structures in the larvae. Developmental modulation of dNSF-2 expression suggests that quantitative changes in the secretory apparatus are important in histogenesis.