A field indoor air measurement of SARS-CoV-2 in the patient rooms of the largest hospital in Iran
A field indoor air measurement of SARS-CoV-2 in the patient rooms of the largest hospital in Iran
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DOI：
10.1016/j.scitotenv.2020.138401
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发表时间：
2020-07-10
影响因子：
9.8
通讯作者：
MokhtariAzad, Talat
中科院分区：
文献类型：
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作者：
Faridi, Sasan;Niazi, Sadegh;MokhtariAzad, Talat
2019冠状病毒病（COVID-19）于2019年底在中国武汉市出现，并迅速蔓延至全球。SARS-CoV-2的主要传播途径存在争议，空气传播途径可能是室内环境中携带病毒的传播途径。到目前为止，还没有证据表明检测到空气传播的严重急性呼吸系统综合征冠状病毒2（SARS- CoV-2），这可能对COVID-19的潜在传播有影响。我们于2020年3月17日在伊朗最大的医院调查了确诊COVID-19的病房的空气。为了收集SARS-CoV-2颗粒，将10个空气样品收集到含有20 mL DMEM、100 μ g/mL链霉素、100 U/mL青霉素和1%消泡剂的无菌标准小型撞击器中1小时。此外，室内颗粒物浓度，CO2，相对湿度和温度记录整个采样期间。从采样器采集的样品中提取病毒RNA，并应用逆转录PCR（RT-PCR）根据病毒基因组序列确认采集样品的阳性。幸运的是，在这项研究中，从确诊的COVID-19患者病床2至5米处采集的所有空气样本均为阴性。尽管我们指出所有的空气样本都是阴性的，但是，我们建议应该使用实际的患者咳嗽，打喷嚏和呼吸气溶胶进行进一步的体内实验，以显示产生空气中大小的载体气溶胶的可能性和这些载体气溶胶中嵌入病毒的存活率。(C)2020爱思唯尔B. V.保留所有权利。
The coronavirus disease 2019 (COVID-19) emerged in Wuhan city, China, in late 2019 and has rapidly spread throughout the world. The major route of transmission of SARS-CoV-2 is in contention, with the airborne route a likely transmission pathway for carrying the virus within indoor environments. Until now, there has been no evidence for detection of airborne severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) and this may have implication for the potential spread of the COVID-19. We investigated the air of patient rooms with confirmed COVID-19 in the largest hospital in Iran, on March 17, 2020. To collect the SARS-CoV-2 particles, ten air samples were collected into the sterile standard midget impingers containing 20 mL DMEM with 100 mu g/mL streptomycin, 100 U/mL penicillin and 1% antifoam reagent for 1 h. Besides, indoor particle number concentrations, CO2, relative humidity and temperature were recorded throughout the sampling duration. Viral RNA was extracted from samples taken from the impingers and Reverse-Transcription PCR (RT-PCR) was applied to confirm the positivity of collected samples based on the virus genome sequence. Fortunately, in this study all air samples which were collected 2 to 5m from the patients' beds with confirmed COVID-19 were negative. Despite we indicated that all air samples were negative, however, we suggest further in vivo experiments should be conducted using actual patient cough, sneeze and breath aerosols in order to show the possibility of generation of the airborne size carrier aerosols and the viability fraction of the embedded virus in those carrier aerosols. (C) 2020 Elsevier B.V. All rights reserved.