DNA Fingerprinting and Genetic Characterization of Anatolian Triticum spp. using AFLP Markers
DNA Fingerprinting and Genetic Characterization of Anatolian Triticum spp. using AFLP Markers
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DOI：
10.1007/s10722-004-7938-3
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发表时间：
2006-04
影响因子：
2
通讯作者：
F. Yildirim;M. Akkaya
中科院分区：
文献类型：
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作者：
F. Yildirim;M. Akkaya
本研究对小麦进行了遗传分析。采用AFLP标记。从每个品种（70个品种）的单个种子获得的所有个体样本中，对6个AFLP选择组合进行了条带存在和不存在的评分；baeoticum (21), T。monococcum (5), T。urartu (16), T。araraticum (7), T。dicoccoides(16)和。dicoccon(5)，产生506个多态AFLP条带。系统发育树显示两个主要集群；一个是由ofT组成的。monococcum (AA)和。baeticum (AA)，另一个簇包括dt。araraticum (AAGG), T。dicoccon (AABB), T。dicoccoides (AABB),和。urartu .T (AA)。乌拉尔图虽然具有二倍体AA基因组，但没有与其他a基因组二倍体如ast、monococumanddt聚集在一起。baeoticum;相反，它与四倍体物种聚集在一起，证实了t。urartuis是A基因组的祖先。讨论了物种内部和物种之间的变异程度。
In this study, genetic analysis ofTriticumspp. was carried out using AFLP markers. Six AFLP selective combinations were scored as presence and absence of bands for all the individual samples obtained from a single seed of each accession (70 accessions);T. baeoticum(21),T. monococcum(5),T. urartu(16),T. araraticum(7),T. dicoccoides(16) andT. dicoccon(5), resulting in 506 polymorphic AFLP bands. The phylogenetic tree showed two major clusters; one was composed ofT. monococcum(AA) andT. baeoticum(AA), and the other cluster includedT. araraticum(AAGG),T. dicoccon(AABB),T. dicoccoides(AABB), andT. urartu(AA).T. urartu, although having a diploid AA genome, did not cluster with other A genome diploids such asT. monococcumandT. baeoticum; instead it clustered together with the tetraploid species, confirming thatT. urartuis the A genome progenitor. The extent of variations within and among species is discussed.