A new method for visualization of gut mucosal cells, describing the enterochromaffin cell in the rat gastrointestinal tract
A new method for visualization of gut mucosal cells, describing the enterochromaffin cell in the rat gastrointestinal tract
复制标题
DOI：
10.1080/00365520500331281
复制
发表时间：
2006-03-01
影响因子：
1.9
通讯作者：
Waldum, HL
中科院分区：
文献类型：
--
作者：
Gustafsson, BI;Bakke, I;Waldum, HL
Objective.肠嗜铬细胞（EC）在胃肠道中作为分泌、运动和感觉的调节者具有重要的功能。EC细胞传统上被描述为瓶形，其底部储存有血清素。作用于顶膜的刺激触发5-羟色胺释放，这反过来激活上皮下感觉神经末梢。为了更好地描述EC细胞的外观，我们开发了一种新的粘膜细胞可视化方法。从Sprague-道利大鼠切除胃、小肠和大肠，然后固定在福尔马林中。将器官外翻并填充链霉蛋白酶溶液。通过从粘膜肌层刮下粘膜收集单个细胞和福尔马林固定的粘膜细胞聚集体。EC细胞通过染色以观察对马槟榔素的免疫反应性。EC细胞有腔状延伸和很长的（可达80 μ M）位于基底的轴突样延伸，有时连接到神经元样结构，其他EC细胞没有或只有短而钝的基底延伸。同时可见分裂的含5-羟色胺EC细胞。这些发现可能会作出重要贡献，进一步了解EC细胞在胃肠道生理功能。这种新的方法也可以很容易地用于更好地显示其他粘膜细胞的形态。
Objective. Enterochromaffin ( EC) cells in the gastrointestinal tract have an important function as regulators of secretion, motility and sensation. The EC cell has traditionally been described as bottle- shaped, with basally located stores of serotonin. Stimuli acting on the apical membrane trigger serotonin release, which in turn activates the subepithelial sensory nerve terminals. To better describe the appearance of EC cells, we developed a new method for visualization of mucosal cells.Material and methods. The stomach, small intestine and large intestine were excised from Sprague- Dawley rats and then fixed in formalin. The organs were everted and filled with pronase solution. Single cells and aggregates of formalin-fixed mucosal cells were collected by scraping the mucosa off the muscularis mucosa. EC cells were visualized by staining for immunoreactivity against serotonin.Results. EC cells with luminal extensions and very long ( up to 80 mu M) basally located axon- like extensions, sometimes connecting to neuron- like structures, were found. Other EC cells had no or only short and blunt basal extensions. Dividing serotonin- containing EC cells were also seen.Conclusions. These findings could make an important contribution towards furthering our understanding of EC cell function in gastrointestinal physiology. This new method can also readily be used to give better visualization of the morphology of other mucosal cells.