Targeted metabolite analysis and antioxidant potential of Rumex induratus.
Targeted metabolite analysis and antioxidant potential of Rumex induratus.
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DOI：
10.1021/jf801385z
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发表时间：
2008-08
影响因子：
6.1
通讯作者：
L. Guerra;C. Pereira;P. Andrade;M. Rodrigues;F. Ferreres;P. G. de Pinho;R. Seabra;P. Valentão
中科院分区：
文献类型：
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作者：
L. Guerra;C. Pereira;P. Andrade;M. Rodrigues;F. Ferreres;P. G. de Pinho;R. Seabra;P. Valentão
对酸模叶水提取物的酚类化合物和有机酸进行了靶向代谢分析，并研究了其对 DPPH(*) 自由基、活性氧、次氯酸和活性氮、一氧化氮 ((*)NO) 的抗氧化活性。这些样本是在不同发育阶段和季节的多个地点采集的，可能是自然发生的，也可能是来自温室培养的。通过高效液相色谱（HPLC）-二极管阵列检测获得酚类成分，并测定了4种羟基肉桂酸衍生物和10种黄酮苷（C-和O-杂苷）。通过 HPLC-UV 确定了有机酸组成，揭示了五种化合物。酚类化合物和有机酸的总量受生长条件和发育阶段的影响。水提取物对所有测试的活性物质均表现出剂量相关的活性。
Targeted metabolite analysis of aqueous extract of Rumex induratus leaves, in terms of phenolic compounds and organic acids, and the study of its antioxidant activity against the DPPH(*) radical, a reactive oxygen species, hypochlorous acid, and a reactive nitrogen species, nitric oxide ((*)NO), were performed. The samples were collected in several locations, spontaneously occurring or from greenhouse culture, at different stages of development and seasons. The phenolic composition was achieved by high-performance liquid chromatography (HPLC)-diode array detection, and four hydroxycinnamic acid derivatives and 10 flavonoid glycosides (C- and O-heterosides) were determined. Organic acids composition was established by HPLC-UV, revealing five compounds. The total amount of phenolic compounds and organic acids were affected by growing conditions and developmental phase. The aqueous extract exhibited a dose-related activity against all tested reactive species.