Myofibrils in Cardiomyocytes Tend to Assemble Along the Maximal Principle Stress Directions.
Myofibrils in Cardiomyocytes Tend to Assemble Along the Maximal Principle Stress Directions.
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心肌细胞中的肌原纤维倾向于沿着最大原则应力方向组装。
DOI：
10.1115/1.4037795
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
KitParker,Kevin
中科院分区：
文献类型：
--
作者：
Yuan,Hongyan;Marzban,Bahador;KitParker,Kevin
心肌组织中自组装肌原纤维的空间组织的机制仍然不完全清楚。通过将细胞建模为弹性固体，在积极的细胞骨架收缩下，我们发现预测的最大主应力方向和体外肌原纤维取向在单个心肌细胞之间有很好的相关性。这意味着肌动球蛋白纤维倾向于沿沿着最大张应力（MTS）方向组装。通过考虑模型中的粘着斑和肌原纤维形成的动力学，我们发现，在成熟与不成熟的心肌细胞中的肌原纤维组织的不同模式可以解释为粘着斑的不同水平的力依赖性重塑的结果。此外，我们将力学模型应用于细胞对，并表明肌原纤维组织可以通过多种因素的组合来调节，包括细胞形状，细胞-基质粘附和细胞-细胞粘附。这一力学模型可指导组织工程心脏的合理设计，因为在体肌原纤维组织的重现对心脏的收缩功能至关重要。
The mechanisms underlying the spatial organization of self-assembled myofibrils in cardiac tissues remain incompletely understood. By modeling cells as elastic solids under active cytoskeletal contraction, we found a good correlation between the predicted maximal principal stress directions and the in vitro myofibril orientations in individual cardiomyocytes. This implies that actomyosin fibers tend to assemble along the maximal tensile stress (MTS) directions. By considering the dynamics of focal adhesion and myofibril formation in the model, we showed that different patterns of myofibril organizations in mature versus immature cardiomyocytes can be explained as the consequence of the different levels of force-dependent remodeling of focal adhesions. Further, we applied the mechanics model to cell pairs and showed that the myofibril organizations can be regulated by a combination of multiple factors including cell shape, cell–substrate adhesions, and cell–cell adhesions. This mechanics model can guide the rational design in cardiac tissue engineering where recapitulating in vivo myofibril organizations is crucial to the contractile function of the heart.