Novel whole body plethysmography system for the continuous characterization of sleep and breathing in a mouse
Novel whole body plethysmography system for the continuous characterization of sleep and breathing in a mouse
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DOI：
10.1152/japplphysiol.00818.2011
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发表时间：
2012-02-01
影响因子：
3.3
通讯作者：
Schwartz, A. R.
中科院分区：
文献类型：
--
作者：
Hernandez, A. B.;Kirkness, J. P.;Schwartz, A. R.
埃尔南德斯 AB、柯克尼斯 JP、史密斯 PL、施耐德 H、波洛茨基 M、理查森 RA、埃尔南德斯 WC、施瓦茨 AR。新型全身体积描记系统，用于连续表征小鼠的睡眠和呼吸。 J Appl Physiol 112: 671-680, 2012。首次发表于 2011 年 12 月 1 日； doi: 10.1152/japplphyol.00818.2011.-睡眠与通气控制的显着改变相关，导致呼吸定时、呼吸模式、通气、咽塌陷和睡眠相关呼吸障碍 (SRBD) 的扰动。小鼠模型为 SRBD 的发病机制提供了深入的了解；然而，尚未开发出在睡眠和清醒期间不受约束的小鼠中获得完整的连续、高保真呼吸、脑电图 (EEG) 和肌电图 (EMG) 信号的方法。我们采用全身体积描记法来连续记录不受约束、未麻醉的小鼠的脑电图、肌电图和呼吸信号。全身体积描记潮气量和气流信号以及呼吸努力的新型无创替代指标（呼吸运动信号）根据同时测量的金标准信号进行了验证。与金标准相比，我们验证了 1) 潮气量（相关性，R-2 = 0.87，P < 0.001；一致性在 1% 以内，P < 0.001）； 2) 吸气气流（相关性，R-2 = 0.92，P < 0.001；一致性在 4% 以内，P < 0.001）； 3) 呼气气流（相关性，R-2 = 0.83，P < 0.001）； 4) 呼吸运动信号（相关性，R-2 = 0.79-0.84，P < 0.001）。然而，与金标准相比，呼气气流信号的幅度有所下降。整合呼吸和脑电图/肌电图信号，我们充分描述了意识清醒、不受约束的小鼠的睡眠和呼吸模式，并证明了新西兰肥胖小鼠的吸气流量受限。我们的方法将促进近交小鼠品系中 SRBD 机制的研究，并提供一个强大的平台来研究环境和药物暴露对睡眠和清醒期间呼吸障碍的影响。
Hernandez AB, Kirkness JP, Smith PL, Schneider H, Polotsky M, Richardson RA, Hernandez WC, Schwartz AR. Novel whole body plethysmography system for the continuous characterization of sleep and breathing in a mouse. J Appl Physiol 112: 671-680, 2012. First published December 1, 2011; doi: 10.1152/japplphysiol.00818.2011.-Sleep is associated with marked alterations in ventilatory control that lead to perturbations in respiratory timing, breathing pattern, ventilation, pharyngeal collapsibility, and sleep-related breathing disorders (SRBD). Mouse models offer powerful insight into the pathogenesis of SRBD; however, methods for obtaining the full complement of continuous, high-fidelity respiratory, electroencephalographic (EEG), and electromyographic (EMG) signals in unrestrained mice during sleep and wake have not been developed. We adapted whole body plethysmography to record EEG, EMG, and respiratory signals continuously in unrestrained, unanesthetized mice. Whole body plethysmography tidal volume and airflow signals and a novel noninvasive surrogate for respiratory effort (respiratory movement signal) were validated against simultaneously measured gold standard signals. Compared with the gold standard, we validated 1) tidal volume (correlation, R-2 = 0.87, P < 0.001; and agreement within 1%, P < 0.001); 2) inspiratory airflow (correlation, R-2 = 0.92, P < 0.001; agreement within 4%, P < 0.001); 3) expiratory airflow (correlation, R-2 = 0.83, P < 0.001); and 4) respiratory movement signal (correlation, R-2 = 0.79-0.84, P < 0.001). The expiratory airflow signal, however, demonstrated a decrease in amplitude compared with the gold standard. Integrating respiratory and EEG/EMG signals, we fully characterized sleep and breathing patterns in conscious, unrestrained mice and demonstrated inspiratory flow limitation in a New Zealand Obese mouse. Our approach will facilitate studies of SRBD mechanisms in inbred mouse strains and offer a powerful platform to investigate the effects of environmental and pharmacological exposures on breathing disturbances during sleep and wakefulness.