A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum
A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum
复制标题
来自植物病原真菌禾谷镰刀菌的低病毒科的一种新病毒
DOI：
10.1016/j.virusres.2013.03.002
复制
发表时间：
2013-06-01
期刊：
影响因子：
5
通讯作者：
Qiu, Dewen
中科院分区：
文献类型：
--
作者：
Wang, Shuangchao;Kondo, Hideki;Qiu, Dewen
从禾谷镰刀菌（Fusariumgraminearum）田间分离物HN 10中纯化得到一个大小约为13 kb的双链RNA（dsRNA）元件，其编码链长13,023个核苷酸（不包括3' poly（A）尾），含有两个不连续的开放阅读框架（ORF A和ORF B）。该基因5'端的ORF A为531 nt，编码176个氨基酸，经BLAST检索，与角孢腐霉低病毒1（Valsa ceratosperma hypovirus 1）和栗疫病菌低病毒4（Cryphonectria hypovirus 4，CHV 4）编码的木瓜蛋白酶样蛋白酶结构域同源性分别为35%和31%。3'端开放阅读框B长11，118 nt，编码一个大的多聚蛋白，包含木瓜蛋白酶样蛋白酶、RNA依赖性RNA聚合酶和RNA解旋酶三个保守结构域。该蛋白与CHV 1（32%）和CHV 2（32%）具有很高的氨基酸同源性。基因组结构和系统发育分析表明，该RNA代表一种新的低病毒，命名为“禾谷镰刀菌低病毒1（FgHV 1）"，与低病毒科中的CHV 1和CHV 2关系密切。病毒的消除没有导致真菌的显著表型改变。(C)2013爱思唯尔有限公司版权所有。
A double-stranded (ds) RNA element, sized at approximately 13 kb pairs, was purified from a field isolate, HN10, of Fusariumgraminearum.The coding strand of the dsRNA was 13,023 nucleotides (nt) long (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORF A and ORF B). The 5' proximal ORF A of 531 nt encoded a protein of 176 amino acids (aa), and a BLAST search showed it to be similar to the putative papain-like protease domains encoded by Valsa ceratosperma hypovirus 1 (35% identity) and Cryphonectria hypovirus 4 (CHV4) (31% identity). The 3' proximal ORF B of 11,118 nt encoded a large polyprotein with three conserved domains, including papain-like protease, RNA-dependent RNA polymerase and RNA helicase domains. The polyprotein shared significant aa identities with CHV1 (32%) and CHV2 (32%). Both the genome organization and phylogenetic analysis suggested that the characterized RNA represented a novel hypovirus, designated "Fusarium graminearum hypovirus 1 (FgHV1)", which was closely related to CHV1 and CHV2 in the Hypoviridae family. Elimination of the virus resulted in no dramatic phenotypic alteration of the fungus. (C) 2013 Elsevier B.V. All rights reserved.