Electricity-Assisted Biological Hydrogen Production from Acetate by Geobacter sulfurreducens
Electricity-Assisted Biological Hydrogen Production from Acetate by Geobacter sulfurreducens
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DOI：
10.1021/es102842p
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发表时间：
2011-01-15
影响因子：
11.4
通讯作者：
Stams, Alfons J. M.
中科院分区：
文献类型：
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作者：
Geelhoed, Jeanine S.;Stams, Alfons J. M.
硫还原地杆菌是微生物燃料电池中众所周知的产电流微生物，它能够以乙酸和氢作为电子供体。本文研究了硫还原g在微生物电解电池（MEC）阴极上作为氢生成生物催化剂的功能。以乙酸为底物，在反电极处还原络合的Fe(III)，在MFC的生物电极室中培养硫还原菌。乙酸耗尽后，生物电极的电极电位逐步降低至-1.0 V /Ag /AgCl基准。观察到负电流的产生，负电流随时间增加，表明生物电极现在起生物阴极的作用。在-0.8 V至-1.0 V的电极电位范围内进行的顶空分析表明，在阴极电位越负的情况下，氢气的产生率越高。随后，在0.8 V和0.65 V的电压下，将G.硫还原物在阳极氧化乙酸和阴极产氢的代谢特性结合在一室无膜mec中。两天后，在0.8 V电压下，电流密度为0.44 A m(-2)，在0.65 V电压下，电流密度为0.22 A m(-2)，阳极和阴极均使用平面碳电极。阴极氢气回收率在0.5 V电压下为23%，在0.9 V电压下为43%。
Geobacter sulfurreducens is a well-known current-producing microorganism in microbial fuel cells, and is able to use acetate and hydrogen as electron donor. We studied the functionality of G. sulfurreducens as biocatalyst for hydrogen formation at the cathode of a microbial electrolysis cell (MEC). Geobacter sulfurreducens was grown in the bioelectrode compartment of a MFC with acetate as the substrate and reduction of complexed Fe(III) at the counter electrode. After depletion of the acetate the electrode potential of the bioelectrode was decreased stepwise to -1.0 V vs Ag/AgCl reference. Production of negative current was observed, which increased in time, indicating that the bioelectrode was now acting as biocathode. Headspace analyses carried out at electrode potentials ranging from -0.8 to -1.0 V showed that hydrogen was produced, with higher rates at more negative cathode potentials. Subsequently, the metabolic properties of G. sulfurreducens for acetate oxidation at the anode and hydrogen production at the cathode were combined in one-compartment membraneless MECs operated at applied voltages of 0.8 and 0.65 V. After two days, current densities were 0.44 A m(-2) at 0.8 V applied voltage and 0.22 A m(-2) at 0.65 V, using flat-surface carbon electrodes for both anode and cathode. The cathodic hydrogen recovery ranged from 23% at 0.5 V applied voltage to 43% at 0.9 V.