Increased cell-free viral DNA in fatal cases of chronic active Epstein-Barr virus infection.
Increased cell-free viral DNA in fatal cases of chronic active Epstein-Barr virus infection.
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慢性活动性 Epstein-Barr 病毒感染致死病例中游离病毒 DNA 增加。
DOI：
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发表时间：
1999
影响因子：
11.8
通讯作者：
Giovanna Tosato
中科院分区：
文献类型：
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作者：
Hirokazu Kanegane;Hiroshi Wakiguchi;C. Kanegane;Takanobu Kurashige;Toshio Miyawaki;Giovanna Tosato
我们研究了33例慢性活动性EB病毒（EBV）感染的性质。研究人群包括14例致命性慢性EBV感染患者和19例非致命性慢性EBV感染患者，以及18例急性EBV诱导的传染性单核细胞增多症患者和10例健康对照。用半定量聚合酶链反应检测患者血清或血浆中的EBV DNA。62%（9/14）的慢性活动性EB病毒感染患者的血清或血浆标本中检出EB病毒DNA。与此相反，只有11%（2/19）的非致命性慢性活动性EBV感染患者和11%（2/18）的传染性单核细胞增多症患者显示EBV DNA。健康对照组均未检测出阳性。无细胞循环EBV DNA可能代表慢性活动性EBV感染的重要特征，并可能提供有用的工具来监测这种疾病的严重程度。
We have studied the nature of Epstein-Barr virus (EBV) infection in 33 patients with chronic active EBV infection. The study population included 14 patients with fatal chronic EBV infection and 19 patients with nonfatal chronic EBV infection, as well as 18 patients with acute EBV-induced infectious mononucleosis and 10 healthy controls. EBV DNA was measured in serum or plasma samples from the patients by semiquantitative polymerase chain reaction-based assay. EBV DNA was detected in serum or plasma samples from 62% (9/14) of patients with fatal chronic active EBV infection. In contrast, only 11% (2/19) of patients with nonfatal chronic active EBV infection and 11% (2/18) of patients with infectious mononucleosis displayed EBV DNA. None of the healthy controls tested positive. Cell-free circulating EBV DNA may represent an important feature of chronic active EBV infection and may provide a useful tool to monitor the severity of this illness.