Genotypic and phenotypic diversity of Streptococcus dysgalactiae strains isolated from clinical and subclinical cases of bovine mastitis
Genotypic and phenotypic diversity of Streptococcus dysgalactiae strains isolated from clinical and subclinical cases of bovine mastitis
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DOI：
10.1016/s0378-1135(96)01221-7
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发表时间：
1996-12-01
影响因子：
3.3
通讯作者：
Jensen, NE
中科院分区：
文献类型：
--
作者：
Aarestrup, FM;Jensen, NE
本研究旨在鉴定从奶牛乳房炎病例中分离的停乳链球菌。采用生物分型（利用塔格糖和山梨醇）和HindIII核糖体分型技术，对来自丹麦100个奶牛群的100个菌株、来自美国宾夕法尼亚州29个奶牛群的29个菌株、7个参考菌株和来自9个乳区的9对菌株组成的18个菌株进行了研究。观察到四种不同的生物型。100株丹麦菌株中只有34株利用塔格糖或山梨糖醇，18株利用塔格糖，15株利用山梨糖醇，1株既利用塔格糖又利用山梨糖醇。相比之下，在美国菌株中，1株（3.4%）既不利用塔格糖也不利用山梨糖醇，20株（69.0%）利用塔格糖，4株（13.8%）利用山梨糖醇，4株（13.8%）既利用塔格糖又利用山梨糖醇。HindIII基因分型在所有菌株中产生了15种不同的类型，其中丹麦菌株中发现了9种不同的类型，美国菌株中发现了8种类型。在丹麦和美国菌株中观察到五种类型。7个参考菌株被分配到5个不同的核糖体型。将三种模式菌株（ATCC 27957、NCTC 4670、NCTC 4335）指定为丹麦和美国菌株中存在的一种类型。核糖分型是最具区分性的分型方法。核糖和生物分型之间的协议进行了观察，尤其是丹麦菌株之间的显着。在每种情况下，发现9对菌株具有相同的核糖和生物型。
This study was conducted to characterize Streptococcus dysgalactiae isolated from cases of bovine mastitis. One hundred strains from 100 dairy herds in Denmark, 29 strains from 29 dairy herds in Pennsylvania, USA, 7 reference strains and 18 strains consisting of 9 paired strains from 9 quarters were investigated using biotyping (utilization of tagatose and sorbitol) and HindIII ribotyping. Four different biotypes were observed. Only 34 of the 100 Danish strains utilized either tagatose or sorbitol, 18 utilized tagatose, 15 sorbitol and 1 both tagatose and sorbitol. In contrast, among the American strains one (3.4%) did neither utilize tagatose or sorbitol, twenty (69.0%) utilized tagatose, four (13.8%) sorbitol and four (13.8%) both tagatose and sorbitol. HindIII ribotyping produced 15 different types among all the strains, 9 different types were found among the Danish strains and 8 types among the American strains. Five types were observed among both the Danish and the American strains. The 7 reference strains were assigned to 5 different ribotypes. Three type strains (ATCC 27957, NCTC 4670, NCTC 4335) were assigned to a type that was present among both the Danish and the American strains. Ribotyping was the most discriminatory typing method. Agreement between ribo- and biotyping was observed, and especially pronounced among the Danish strains. The 9 paired strains were in each case found to have identical ribo- and biotype.