Characterization of stromal vascular fraction and adipose stem cells from subcutaneous, preperitoneal and visceral morbidly obese human adipose tissue depots.
Characterization of stromal vascular fraction and adipose stem cells from subcutaneous, preperitoneal and visceral morbidly obese human adipose tissue depots.
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DOI：
10.1371/journal.pone.0174115
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Baptista LS
中科院分区：
文献类型：
--
作者：
Silva KR;Côrtes I;Liechocki S;Carneiro JR;Souza AA;Borojevic R;Maya-Monteiro CM;Baptista LS
肥胖的病理状况伴随着功能失调的脂肪组织。我们假设皮下、腹膜前和内脏肥胖腹部白色脂肪组织库可能具有具有不同组成的基质血管部分（SVF）和脂肪干细胞（ASC），这将不同地解释肥胖的发病机制。为了评估 SVF 亚群的分布，在减肥手术期间收集了病态肥胖女性（n = 12，BMI：46.2±5.1 kg/m2）的皮下、腹膜前和内脏脂肪组织样本，进行酶消化并通过流式细胞仪进行分析（n = 12）。对所有储存库的 ASC 的形态、表面表达、诱导后积累脂质的能力和细胞因子分泌进行了评估 (n = 3)。在皮下储库的 SVF 中发现了高含量的前脂肪细胞 (p = 0.0178)。来自三个储存库的 ASC 具有相似的成纤维细胞形态，具有均匀的 CD34、CD146、CD105、CD73 和 CD90 表达。来自内脏库的 ASC 分泌最高水平的 IL-6、MCP-1 和 G-CSF (p = 0.0278)。有趣的是，在脂质积累刺激下，腹膜前 ASC 显示所有分泌细胞因子的水平最低，脂联素除外，脂联素水平有所增强 (p = 0.0278)。来自腹膜前脂肪组织的 ASC 显示出较低的促炎特性，尽管它是内部脂肪库。相反，来自内脏脂肪组织的 ASC 最具促炎性。因此，来自皮下、内脏和腹膜前脂肪库的 ASC 可能对肥胖的慢性炎症情况有不同的影响。
The pathological condition of obesity is accompanied by a dysfunctional adipose tissue. We postulate that subcutaneous, preperitoneal and visceral obese abdominal white adipose tissue depots could have stromal vascular fractions (SVF) with distinct composition and adipose stem cells (ASC) that would differentially account for the pathogenesis of obesity. In order to evaluate the distribution of SVF subpopulations, samples of subcutaneous, preperitoneal and visceral adipose tissues from morbidly obese women (n = 12, BMI: 46.2±5.1 kg/m2) were collected during bariatric surgery, enzymatically digested and analyzed by flow cytometry (n = 12). ASC from all depots were evaluated for morphology, surface expression, ability to accumulate lipid after induction and cytokine secretion (n = 3). A high content of preadipocytes was found in the SVF of subcutaneous depot (p = 0.0178). ASC from the three depots had similar fibroblastoid morphology with a homogeneous expression of CD34, CD146, CD105, CD73 and CD90. ASC from the visceral depot secreted the highest levels of IL-6, MCP-1 and G-CSF (p = 0.0278). Interestingly, preperitoneal ASC under lipid accumulation stimulus showed the lowest levels of all the secreted cytokines, except for adiponectin that was enhanced (p = 0.0278). ASC from preperitoneal adipose tissue revealed the less pro-inflammatory properties, although it is an internal adipose depot. Conversely, ASC from visceral adipose tissue are the most pro-inflammatory. Therefore, ASC from subcutaneous, visceral and preperitoneal adipose depots could differentially contribute to the chronic inflammatory scenario of obesity.