Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co-factor for epidermal growth factor in cell culture
Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co-factor for epidermal growth factor in cell culture
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DOI：
10.1016/s0014-5793(97)00386-4
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发表时间：
1997-05-12
期刊：
影响因子：
3.5
通讯作者：
Takahara, H
中科院分区：
文献类型：
--
作者：
Kawada, A;Hiura, N;Takahara, H
对参与调节植物发育和防御过程的低聚糖进行了测试，以确定其增强人类角质形成细胞增殖的能力。藻酸盐寡糖的混合物显着刺激角质形成细胞的生长和[H-3]胸苷的摄取在表皮生长因子（EGP）的存在下。该活性与牛垂体提取物相当，牛垂体提取物是角质形成细胞培养物中的常见补充物，并且添加BPE诱导的刺激。鉴定了混合物中最有效的寡糖，并确定了其化学结构。这些发现证明了藻酸盐寡糖在角质形成细胞生长中的新活性，并表明可能是角质形成细胞培养基中EGF依赖性刺激的辅因子。(C)1997年欧洲生物化学学会联合会。
Oligosaccharides, involved in regulation of plant developmental and defensive processes, were tested to determine their ability to enhance proliferation of human keratinocytes. A mixture of alginate oligosaccharides remarkably stimulated keratinocyte growth and [H-3]thymidine uptake in the presence of epidermal growth factor (EGP). The activity was comparable to bovine pituitary extract, a common complement in keratinocyte culture, and additive on BPE-induced stimulation. The most effective oligosaccharide in the mixture was identified and its chemical structure was determined. These findings demonstrate a novel activity of alginate oligosaccharide(s) in keratinocyte growth and suggest a possible co-factor for EGF-dependent stimulation in medium for keratinocytes. (C) 1997 Federation of European Biochemical Societies.