Fertilization-induced changes in the vitelline envelope of echinoderm and amphibian eggs: self-assembly of an extracellular matrix.
Fertilization-induced changes in the vitelline envelope of echinoderm and amphibian eggs: self-assembly of an extracellular matrix.
复制标题
受精引起的棘皮动物和两栖动物卵卵黄包膜的变化：细胞外基质的自组装。
DOI：
10.1002/jemt.1060170305
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Chandler,DE
中科院分区：
文献类型：
--
作者：
Larabell,C;Chandler,DE
未受精的海胆卵的表面被卵黄层（VL）覆盖，这是一种含有精子受体的纤维细胞外基质。在受精时，皮质颗粒胞吐作用释放酶和结构蛋白，导致VL升高并被重塑为机械和化学上坚韧的受精包膜。这种包膜可以防止精子进一步渗透，并在早期发育过程中保护胚胎。一个更厚，更复杂的卵黄包膜包围非洲爪蟾laevisegg。这种纤维状的外套在受精时也会重新构造，以产生一个无法穿透的精子屏障。这些受精包膜的自组装过程中发生的生化步骤进行审查，并发生的超微结构的变化，如在铂复制品的速冻，深蚀刻，和旋转阴影的鸡蛋，进行了说明。
The surface of the unfertilized sea urchin egg is covered by the vitelline layer (VL), a fibrous extracellular matrix that contains receptors for sperm. At fertilization, cortical granule exocytosis releases enzymes and structural proteins that cause the VL to elevate and become remodelled into the mechanically and chemically tough fertilization envelope. This envelope prevents further penetration of sperm and protects the embryo during early development. A thicker, more complex vitelline envelope surrounds theXenopus laevisegg. This fibrous coat is also restructured at fertilization to produce an impenetrable barrier to sperm. The biochemical steps that occur during self‐assembly of these fertilization envelopes are reviewed, and the ultrastructural changes that occur, as seen in platinum replicas of quick‐frozen, deep‐etched, and rotaryshadowed eggs, are illustrated.