AGROBACTERIUM - GENETIC-STUDIES ON AGROCIN-84 PRODUCTION AND THE BIOLOGICAL-CONTROL OF CROWN GALL
AGROBACTERIUM - GENETIC-STUDIES ON AGROCIN-84 PRODUCTION AND THE BIOLOGICAL-CONTROL OF CROWN GALL
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DOI：
10.1016/0048-4059(79)90082-1
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
SCHELL, J
中科院分区：
文献类型：
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作者：
ELLIS, JG;KERR, A;SCHELL, J
通过构建新的产菌菌株，研究了细菌素农杆菌素 84 在生物防治冠瘿病中的作用。鉴定了编码能够产生农杆菌素84的小质粒，并通过接合将其转移至受体菌株。使用了两种供体菌株；无毒株84和强毒株398。通过基因操作将后一株中的农杆菌素产生和毒力分离，并产生无毒的、产生农杆菌素的衍生物。产生农杆菌素84的无毒转接合子作为生物防治剂的有效性各不相同。有证据表明，这可能是由于接种地点不同菌株的生长速率不同所致。
The role of the bacteriocin, agrocin 84, in the biological control of crown gall was investigated by constructing new bacteriocinogenic strains. A small plasmid which codes for the ability to produce agrocin 84 was identified and transferred to recipient strains by conjugation. Two donor strains were used; the avirulent strain 84 and the virulent strain 398. Agrocin production and virulence in the latter strain were separated by genetic manipulation and avirulent, agrocin producing derivatives were produced. Avirulent transconjugants which produced agrocin 84 varied in their effectiveness as biological control agents. Evidence is presented that this was probably due to variation in growth rate of the different strains in the inoculation sites.