ELEMENTS OF THE YEAST PHEROMONE RESPONSE PATHWAY REQUIRED FOR FILAMENTOUS GROWTH OF DIPLOIDS
ELEMENTS OF THE YEAST PHEROMONE RESPONSE PATHWAY REQUIRED FOR FILAMENTOUS GROWTH OF DIPLOIDS
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DOI：
10.1126/science.8259520
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发表时间：
1993-12-10
期刊：
影响因子：
56.9
通讯作者：
FINK, GR
中科院分区：
文献类型：
--
作者：
LIU, HP;STYLES, CA;FINK, GR
从受体到下游靶点的外部信号传递通常由一组保守的蛋白激酶介导，这些蛋白激酶按顺序起作用（激酶级联）。在酿酒酵母的单倍体菌株中，由肽信息素启动的信号通过这种激酶级联传递到转录因子STE12，这是许多交配特异性基因表达所必需的。在这里，它表明，在二倍体一些相同的激酶和STE12所需的丝状生长，但信息素受体和鸟苷三磷酸结合蛋白是不需要的丝状体的形成。因此，类似的激酶级联在单倍体和二倍体中被不同的信号激活，并介导两种细胞类型中不同的发育结果。
Transmission of an external signal from receptors to downstream targets is often mediated by a conserved set of protein kinases that act in sequence (a kinase cascade). In haploid strains of Saccharomyces cerevisiae, a signal initiated by peptide pheromones is transmitted through this kinase cascade to a transcription factor STE12, which is required for the expression of many mating-specific genes. Here it was shown that in diploids some of the same kinases and STE12 are required for filamentous growth, but the pheromone receptors and guanosine triphosphate-binding protein are not required for filament formation. Thus, a similar kinase cascade is activated by different signals in haploids and diploids and mediates different developmental outcomes in the two cell types.