Issues surrounding standard cytotoxicity testing for assessing activity of non-covalent DNA-binding metallo-drugs.
Issues surrounding standard cytotoxicity testing for assessing activity of non-covalent DNA-binding metallo-drugs.
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DOI：
10.1039/b927129p
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发表时间：
2010-03
影响因子：
4
通讯作者：
A. J. Pope;Christopher Bruce;B. Kysela;M. J. Hannon
中科院分区：
文献类型：
--
作者：
A. J. Pope;Christopher Bruce;B. Kysela;M. J. Hannon
研究通常用于评估新型化合物（特别是非共价 DNA 结合剂）的体外细胞毒性的方法，发现这些方法可能并不合适。抗癌活性水平不仅取决于孵育时间，还取决于所用药物化合物的绝对量（摩尔数），而不是浓度。
Investigating the methods commonly used to evaluate in vitro cytotoxicity of novel compounds, specifically non-covalent DNA binders, identifies that these methods may not be appropriate. The level of anticancer activity depends not only on the incubation time but also on the absolute amount (number of moles) of drug compound applied, rather than the concentration.