A CBL-interacting protein kinase, AcCIPK18, from Ananas comosus regulates tolerance to salt, drought, heat stress and Sclerotinia sclerotiorum infection in Arabidopsis
A CBL-interacting protein kinase, AcCIPK18, from Ananas comosus regulates tolerance to salt, drought, heat stress and Sclerotinia sclerotiorum infection in Arabidopsis
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DOI：
10.1016/j.envexpbot.2021.104728
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发表时间：
2022-02-01
影响因子：
5.7
通讯作者：
Qin, Yuan
中科院分区：
文献类型：
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作者：
Aslam, Mohammad;Fakher, Beenish;Qin, Yuan
在植物中，类钙调神经磷酸酶B蛋白(CBL)及其相互作用的蛋白激酶(CIPKs)在调节对包括生物和非生物胁迫在内的各种细胞外信号的反应中发挥重要作用。几个CIPK基因已经在不同的植物物种中得到了功能鉴定。然而，到目前为止，菠萝CIPK的功能尚未被研究，这限制了其在分子育种中的潜在应用。在这里，我们对菠萝AcCIPK18进行了功能鉴定，并验证了它在盐、干旱、高温胁迫和免疫中的作用。生物信息学研究和系统发育分析表明，AcCIPK18与拟南芥的CIPK6同源。表达分析表明，AcCIPK18受到盐、渗透压、热和ABA的诱导。此外，异源表达的AcCIPK18提高了拟南芥对盐、干旱和高温胁迫的耐受性，但对真菌侵染表现出敏感性。我们发现，AcCIPK18的过表达通过改变几个ABA信号基因的表达来改变ROS的动态平衡。这些结果为AcCIPK18参与多种胁迫反应提供了证据，并主张AcCIPK18可以作为一个潜在的候选基因来提高对多种非生物胁迫的耐受性。
In plants, calcineurin B-like proteins (CBLs) and their interacting kinase, CBL-interacting protein kinases (CIPKs), perform essential roles in mediating responses to various extracellular cues, including biotic and abiotic stresses. Several CIPK genes have been functionally characterized in different plant species. However, to date, the functions of pineapple CIPKs have not been investigated, which limits their potential use in molecular breeding. Here we functionally characterized an AcCIPK18 of pineapple and verified its role in salt, drought, heat stresses and immunity. Bioinformatics studies and phylogenetic analysis showed that AcCIPK18 is homologous to CIPK6 of Arabidopsis. Expression analysis shows that AcCIPK18 gets induced by salt, osmotic, heat and ABA. Besides, the ectopic expression of AcCIPK18 increased tolerance to salt, drought and heat stress but displayed sensitivity against fungal infection in Arabidopsis. We found that overexpression of AcCIPK18 changes the ROS homeostasis by altering the expression of several ABA signaling genes. These results provide evidence for the involvement of AcCIPK18 in multiple stress responses and advocates that AcCIPK18 could be used as a potential candidate gene for improving tolerance against multiple abiotic stress.