Direct and indirect control of oral ectoderm regulatory gene expression by Nodal signaling in the sea urchin embryo
Direct and indirect control of oral ectoderm regulatory gene expression by Nodal signaling in the sea urchin embryo
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DOI：
10.1016/j.ydbio.2012.06.022
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发表时间：
2012-09-15
影响因子：
2.7
通讯作者：
Davidson, Eric H.
中科院分区：
文献类型：
--
作者：
Li, Enhu;Materna, Stefan C.;Davidson, Eric H.
根据早期的工作，Nodal信号通路被认为是海胆胚胎中口腔外胚层规范的重要中介，也是流产外胚层规范的间接中介。随着Nodal配体在未来的口腔外胚层中的表达，在这个区域内发生了一系列调控基因的激活，这一序列直接或间接地依赖于Nodal基因的表达。在这里，我们描述了额外的调控基因，有助于口腔外胚层的调节状态在规格期间，并显示了它们的空间表达如何动态变化的发育过程。通过系统范围的扰动分析，我们显着改进了对口腔外胚层调控基因之间上位关系的现有认识。从这些研究中，出现了不同的电路，直接或间接地将下游调控基因与Nodal信号联系在一起。一个关键的中介监管机构是NOT基因，它的作用以前没有被察觉。除了激活几个早先被描述为Nodal信号靶标的基因外，NOT基因产物还作用于抑制其他口腔外胚层基因，对胚胎口腔外胚层的双边空间组织起关键作用。(C)2012 Elsevier Inc.保留所有权利。
The Nodal signaling pathway is known from earlier work to be an essential mediator of oral ectoderm specification in the sea urchin embryo, and indirectly, of aboral ectoderm specification as well. Following expression of the Nodal ligand in the future oral ectoderm during cleavage, a sequence of regulatory gene activations occur within this territory which depend directly or indirectly on nodal gene expression. Here we describe additional regulatory genes that contribute to the oral ectoderm regulatory state during specification in Strongylocentrotus purpuratus, and show how their spatial expression changes dynamically during development. By means of system wide perturbation analyses we have significantly improved current knowledge of the epistatic relations among the regulatory genes of the oral ectoderm. From these studies there emerge diverse circuitries relating downstream regulatory genes directly and indirectly to Nodal signaling. A key intermediary regulator, the role of which had not previously been discerned, is the not gene. In addition to activating several genes earlier described as targets of Nodal signaling, the not gene product acts to repress other oral ectoderm genes, contributing crucially to the bilateral spatial organization of the embryonic oral ectoderm. (C) 2012 Elsevier Inc. All rights reserved.