Evaluation of the phototoxic potential of plants used in oriental medicine
Evaluation of the phototoxic potential of plants used in oriental medicine
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DOI：
10.1016/j.jep.2009.09.058
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发表时间：
2010-01-08
影响因子：
5.4
通讯作者：
Kim, Tae Heung
中科院分区：
文献类型：
--
作者：
Bark, Ki-Min;Heo, Eun Phil;Kim, Tae Heung
研究目的：光毒性可能是有害的，也可能是有益的。然而，东方药用植物的光毒性仍是一个尚未得到充分研究的领域。本研究的目的就是填补这一空白。材料和方法：使用光溶血和白色念珠菌试验在体外检查东方药用植物的光毒性潜力。十七种药用植物 [石菖蒲 (ACC)、人参 C.A. (PAG)、桔梗 (PLG)、白兰地 (ATJ)、苍耳 (XAS)、薯蓣 (DIB)、知母 (ANA)、黄精 (PSR)、三叶花 (COT)、无花果 (FIC)、白屈菜 (FIC)。选择asiaticum (CMA)、Pulsatilla koreana (PUK)、Agrimonia pilosa (AGP)、Zanthoxylum schinifolium (ZAS)、Angelica gigas (ANG)、Ledebouriella seseloides (LES)和Cnidium officinale (CNO)]是因为它们在我们之前对62种植物的一项研究中表现出强烈的荧光。我们进一步评估了小鼠体内的光毒性。 0.75 mL/kg 苍耳（XAS，（原文如此））种子油，或 1.25 mL/kg 白术（ATJ，（原文如此））、Chelidium majus var. 的提取溶液。给予积雪草（CMA，（原文如此））、Zanthoxylum schinifolium（ZAS，（原文如此））和Ledeboutiella seseloides（LES，（原文如此））一次，并评估晒伤水肿、晒伤细胞的形成、表皮朗格汉斯细胞的减少以及UVA照射对接触过敏的局部抑制。结果：除COT外，测试的17种植物中，有16种显示显着光溶血作用，其中 5 种表现出光毒性杀死白色念珠菌。然后在小鼠身上研究了使用这 5 种植物的东方药物的光毒性。这5种植物增加了晒伤水肿和晒伤细胞的形成，并通过UVA照射减少表皮朗格汉斯细胞和接触超敏反应来抑制局部免疫反应。结论：超过四分之一的东方药用植物可能具有光毒性，通过吸收和荧光光谱测量的强荧光可以更容易地筛选光毒性。另一方面，植物的光毒性也可用于治疗。有必要进一步研究从活的和干燥的东方药用植物中提取的活性成分的光毒性。 (C) 2009 Elsevier Ireland Ltd. 保留所有权利。
Aim of the study: Phototoxicity can be either harmful or beneficial. Yet the phototoxicity of oriental medicinal plants is an understudied area. The purpose of this study is to fill in this gap.Materials and methods: The phototoxic potential of oriental medicinal plants was examined in vitro using photohemolysis and the Candida albicans test. Seventeen medicinal plants [Acorus gramineus (ACC), Panax ginseng C.A. (PAG), Platycodon grandiflorum (PLG), Aractylodes japonica (ATJ), Xanthium strumarium (XAS), Dioscorea batatas (DIB), Anemarrhena asphodeloides (ANA), Polygonatum sibiricum Red (PSR), Cocculus trilobus (COT), Ficus carica (FIC), Chelidonium majus var. asiaticum (CMA), Pulsatilla koreana (PUK), Agrimonia pilosa (AGP), Zanthoxylum schinifolium (ZAS), Angelica gigas (ANG), Ledebouriella seseloides (LES), and Cnidium officinale (CNO)] were selected because they showed strong fluorescence in one of our previous studies of 62 plants. We further evaluated in vivo phototoxicity in mice. 0.75 mL/kg of seed oil for Xanthium strumarium (XAS, (sic)), or 1.25 mL/kg of extracted solutions of Atractylodes japonica (ATJ, (sic)), Chelidonium majus var. asiaticum (CMA, (sic)), Zanthoxylum schinifolium (ZAS, (sic)), and Ledeboutiella seseloides (LES, (sic)) were given once, and evaluated for sunburn edema, formation of sunburn cell, decrease of epidermal Langerhans cells and local suppression of contact hypersensitivity by UVA irradiation.Results: Sixteen out of the 17 plants tested except COT showed significant photohemolysis, and 5 of those exhibited phototoxic killing of Candida albicans. The phototoxicity of oriental medicines using those 5 plants was then studied in mice. The 5 plants increased sunburn edema and formation of sunburn cell, and suppressed immune responses locally by decreasing epidermal Langerhans cells and contact hypersensitivity by UVA irradiation.Conclusions: More than a quarter of oriental medicinal plants can be phototoxic, and strong fluorescence measured by absorption and fluorescence spectra can be an easier way to screen for phototoxicity. On the other hand, the phototoxicity of the plants may also be used therapeutically. Further studies regarding the phototoxicity of active components extracted from both live and dried oriental medicinal plants are necessary. (C) 2009 Elsevier Ireland Ltd. All rights reserved.