snail gene expression in the medaka, Oryzias latipes
snail gene expression in the medaka, Oryzias latipes
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DOI：
10.1016/j.gep.2010.11.003
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发表时间：
2011-03-01
影响因子：
1.2
通讯作者：
Schartl, Manfred
中科院分区：
文献类型：
--
作者：
Liedtke, Daniel;Erhard, Isabell;Schartl, Manfred
Snail转录因子在脊椎动物胚胎发育过程中发挥着重要作用。它们通常参与神经嵴发育、上皮-间充质转化和癌症进展期间的细胞迁移过程。对snai基因家族成员在不同脊椎动物物种中的表达进行比较研究，有望有助于更好地理解它们在发育过程中的作用，并反映它们的进化历史。为了研究和比较snai基因在第二种主要鱼类模型中的表达模式，我们使用了青鳉鱼（Oryzias latipes），一种与斑马鱼互补的硬骨鱼模型。我们确定了三个snai基因家族成员，snai 1a，snai 1b和snai 2。系统发育和同线性分析表明，所有家庭成员的其他脊椎动物snai基因的密切相关。令人惊讶的是，在青鳉中没有发现snai 3的同源物，尽管这种基因存在于斑马鱼和河豚中。在这里，我们表明，虽然大多数青鳉基因的表达域与斑马鱼相当，但个体旁系同源物对整体模式的贡献在两种硬骨鱼之间不同，并表明谱系特异性表达改组。(C)2010爱思唯尔有限公司版权所有。
Snail transcription factors have prominent roles during embryonic development of vertebrates. They are often involved in cell migration processes during neural crest development, epithelial-mesenchymal transition and cancer progression. Comparative expression studies of snai gene family members in different vertebrate species are expected to contribute to a better understanding of their roles during development and reflect their evolutionary history. To investigate and to compare the expression patterns of snai genes in a second main fish model we used the medaka fish (Oryzias latipes), a complementary teleost model to zebrafish. We identified three snai gene family members, snai1a, snai1b and snai2. Phylogenetic and synteny analysis show a close relatedness of all family members to other vertebrate snai genes. Surprisingly, no homologue of snai3 could be identified in medaka, although this gene is present in zebrafish and the puffer fishes. Here we demonstrate that while most expression domains of medaka snai genes are comparable to zebrafish, the contribution of the individual paralogs to the overall pattern differs between the two teleosts and indicate lineage specific expression shuffling. (C) 2010 Elsevier B.V. All rights reserved.