Global response of Saccharomyces cerevisiae to an alkylating agent
Global response of Saccharomyces cerevisiae to an alkylating agent
复制标题
DOI：
10.1073/pnas.96.4.1486
复制
发表时间：
1999-02-16
影响因子：
11.1
通讯作者：
Samson, LD
中科院分区：
文献类型：
--
作者：
Jelinsky, SA;Samson, LD
DNA芯片技术可以同时研究代表整个基因组的酿酒酵母基因转录水平的大约对环境变化的反应。通过使用带有寡核苷酸阵列的芯片，我们表明，暴露于烷基化剂甲基甲磺酸甲酯后，大约增加到325个基因转录水平，并且大约降低至76个。在已经被DNA损害剂诱导的21个基因中，可以将18个在此数据集中评分为诱导性，而且令人惊讶的是，大多数新鉴定的诱导基因的诱导诱导甚至比这18个。我们检查了。 42响应式和8个无反应性ORF通过常规北印迹，其中40个ORF如DNA芯片分析所做的，其中48个ORF响应，大小显示相关系数为0.79。响应基因失败了几个预期和许多意外类别。提出了诱导程序以消除和替代烷基化蛋白质的证据。
DNA chip technology enables simultaneous examination of how approximate to 6,200 Saccharomyces cerevisiae gene transcript levels, representing the entire genome, respond to environmental change. By using chips bearing oligonucleotide arrays, we show that, after exposure to the alkylating agent methyl methanesulfonate, approximate to 325 gene transcript levels are increased and approximate to 76 are decreased. Of the 21 genes that already were known to be induced by a DNA-damaging agent, 18 can be scored as inducible in this data set, and surprisingly, most of the newly identified inducible genes are induced even more strongly than these 18. We examined 42 responsive and 8 nonresponsive ORFs by conventional Northern blotting, and 48 of these 50 ORFs responded as they did by DNA chip analysis, with magnitudes displaying a correlation coefficient of 0.79. Responsive genes fail into several expected and many unexpected categories. Evidence for the induction of a program to eliminate and replace alkylated proteins is presented.